Park, Sunmi Son, Min Jeong Yook, Chang-Soo Jin, Changbae Lee, Yong Sup Kim, Hyoung Ja 2024-01-20T10:01:16Z 2024-01-20T10:01:16Z 2021-09-04 2014-05 0031-9422 https://pubs.kist.re.kr/handle/201004/126854 An ethyl acetate fraction of the aerial parts of Caryopteris incana (Verbenaceae) showed potent cytoprotective effects against damage to HepG2 cells induced by tert-butylhydroperoxide (t-BHP). To search for hepatoprotective components of C incana, various chromatographic separations of the ethyl acetate soluble fraction of C incana led to isolation of three phenylpropanoid glycosides, 6'''-O-feruloylincanoside D. 6'''-O-sinapoylincanoside D and caryopteroside, and two iridoid glycosides, incanides A and B, together with 17 known compounds. Structures of these compounds were determined by spectroscopic analyses. The absolute stereochemistry of the caryopteroside was established with the help of circular dichroism data and in comparison with literature data. All isolated substances were determined for their cytoprotective effects against t-BHP-induced toxicity in HepG2 cells. Among the tested compounds, 6'-O-caffeoylacteoside exhibited the most potent cytoprotective activity with an IC50 value of 0.8 +/- 0.1 mu M against t-BHP-induced toxicity. Structure-activity relationships of the assay results indicated an important role of the catechol moiety in phenylpropanoid, iridoid and flavonoid derivatives in eliciting cytoprotective effects. (C) 2014 Elsevier Ltd. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD IRIDOID GLUCOSIDES PHENYLPROPANOID GLYCOSIDES PHENYLETHANOID GLYCOSIDES CYTOTOXICITY ASSAY LIPID-PEROXIDATION PHENOLIC-COMPOUNDS OXIDATIVE STRESS RAT-LIVER APOPTOSIS HEPATOCYTES Chemical constituents from aerial parts of Caryopteris incana and cytoprotective effects in human HepG2 cells Article 10.1016/j.phytochem.2014.02.008 1 PHYTOCHEMISTRY, v.101, pp.83 - 90 PHYTOCHEMISTRY 101 83 90 scie scopus 000341800200009 2-s2.0-84897107374 Biochemistry & Molecular Biology Plant Sciences Biochemistry & Molecular Biology Plant Sciences Article IRIDOID GLUCOSIDES PHENYLPROPANOID GLYCOSIDES PHENYLETHANOID GLYCOSIDES CYTOTOXICITY ASSAY LIPID-PEROXIDATION PHENOLIC-COMPOUNDS OXIDATIVE STRESS RAT-LIVER APOPTOSIS HEPATOCYTES Caryopteris incana (Thunb.) Miq. Verbenaceae Phenylpropanoids Iridoids Cytoprotection tert-Butylhydroperoxide (t-BHP) HepG2 cell