Park, Sung-Jin Yeo, Hock Chuan Kang, Nam-Young Kim, Hanjo Lin, Joyce Ha, Hyung-Ho Vendrell, Marc Lee, Jun-Seok Chandran, Yogeswari Lee, Dong-Yup Yun, Seong-Wook Chang, Young-Tae 2024-01-20T10:01:30Z 2024-01-20T10:01:30Z 2021-08-31 2014-05 1873-5061 https://pubs.kist.re.kr/handle/201004/126865 A better understanding of the cellular and molecular mechanisms involved in the reprogramming of somatic cells is essential for further improvement of induced pluripotent stem (iPS) cell technology. In this study, we enriched for cells actively undergoing reprogramming at different time points by sorting the cells stained with a stem cell-selective fluorescent chemical probe CDy1 for their global gene expression analysis. Day-to-day comparison of differentially expressed genes showed highly dynamic and transient gene expressions during reprogramming, which were largely distinct from those of fully-reprogrammed cells. An unbiased analysis of functional regulation indicated robust modulation of concurrent programs at critical junctures. Globally, transcriptional programs involved in cell proliferation, morphology and signal transduction were instantly triggered as early as 3 days-post-infection to prepare the cell for reprogramming but became somewhat muted in the final iPS cells. On the other hand, the highly coordinated metabolic reprogramming process was more gradually activated. Subsequent network analysis of differentially expressed genes indicated PDGF-BB as a core player in reprogramming which was verified by our gain- and loss-of-function experiments. As such, our study has revealed previously-unknown insights into the mechanisms of cellular reprogramming. (C) 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/3.0/). English ELSEVIER SCIENCE BV PLURIPOTENT STEM-CELLS SOMATIC-CELLS GROWTH-FACTOR SELF-RENEWAL IPS CELLS PATHWAY SPHINGOSINE-1-PHOSPHATE MAINTENANCE PLATFORM ROLES Mechanistic elements and critical factors of cellular reprogramming revealed by stepwise global gene expression analyses Article 10.1016/j.scr.2014.03.002 1 STEM CELL RESEARCH, v.12, no.3, pp.730 - 741 STEM CELL RESEARCH 12 3 730 741 scie scopus 000342286900013 2-s2.0-84898646262 Cell & Tissue Engineering Biotechnology & Applied Microbiology Cell Biology Cell Biology Biotechnology & Applied Microbiology Article PLURIPOTENT STEM-CELLS SOMATIC-CELLS GROWTH-FACTOR SELF-RENEWAL IPS CELLS PATHWAY SPHINGOSINE-1-PHOSPHATE MAINTENANCE PLATFORM ROLES