Kim, Myungsuk Lim, Sue Ji Kang, Suk Woo Um, Byung-Hun Nho, Chu Won 2024-01-20T10:01:31Z 2024-01-20T10:01:31Z 2021-09-05 2014-04-30 0021-8561 https://pubs.kist.re.kr/handle/201004/126866 Aceriphyllum rossii contains an abundant source of natural flavonoids with potential antioxidant, anticancer and anti-inflammatory properties. However, the effect of A. rossii extract (ARE) on immunoglobulin E(IgE)-mediated allergic responses remains unknown. In the present study, the effects of ARE and its active compounds, quercetin and kaempferol, on IgE-mediated rat basophilic leukemia mast cell activation and passive cutaneous anaphylaxis (PCA) were investigated. ARE, quercetin, and kaempferol inhibited secretion of beta-hexosaminidase and histamine, and reduced the production and mRNA expression of interleukin-4 and tumor necrosis factor-a. ARE also decreased the production of prostaglandin E-2 and leukotriene B, and expression of cyclooxygenase 2 and 5-lipoxygenase. Furthermore, ARE, quercetin, and kaempferol inhibited IgE-mediated phosphorylation of Syk, phospholipase C gamma, protein kinase C (PKC)mu, and the mitogen-activated protein kinases, extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase. ARE, quercetin, and kaempferol markedly suppressed mast cell-dependent PCA in IgE-sensitized mice. These results indicate that ARE and its active constituents, quercetin and kaempferol, may be a useful therapy for immediate-type hypersensitivity. English AMER CHEMICAL SOC RELEASE RBL-2H3 PROTEIN ALPHA CYCLOOXYGENASE-2 DEGRANULATION PATHWAY ANTIGEN ACID Aceriphyllum rossii Extract and Its Active Compounds, Quercetin and Kaempferol Inhibit IgE-mediated Mast Cell Activation and Passive Cutaneous Anaphylaxis Article 10.1021/jf405486c 1 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.62, no.17, pp.3750 - 3758 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62 17 3750 3758 scie scopus 000335369100013 2-s2.0-84899767337 Agriculture, Multidisciplinary Chemistry, Applied Food Science & Technology Agriculture Chemistry Food Science & Technology Article RELEASE RBL-2H3 PROTEIN ALPHA CYCLOOXYGENASE-2 DEGRANULATION PATHWAY ANTIGEN ACID quercetin kaempferol Aceriphyllum rossii IgE-mediated allergic diseases RBL-2H3 mast cells