Park, SungJun Park, Hye Hun Kim, Sung Yeon Kim, Su Jung Woo, Kyoungja Ko, GwangPyo 2024-01-20T10:02:23Z 2024-01-20T10:02:23Z 2021-09-05 2014-04 0099-2240 https://pubs.kist.re.kr/handle/201004/126909 Silver nanoparticles (AgNPs) are considered to be a potentially useful tool for controlling various pathogens. However, there are concerns about the release of AgNPs into environmental media, as they may generate adverse human health and ecological effects. In this study, we developed and evaluated a novel micrometer-sized magnetic hybrid colloid (MHC) decorated with variously sized AgNPs (AgNP-MHCs). After being applied for disinfection, these particles can be easily recovered from environmental media using their magnetic properties and remain effective for inactivating viral pathogens. We evaluated the efficacy of AgNP-MHCs for inactivating bacteriophage phi X174, murine norovirus (MNV), and adenovirus serotype 2 (AdV2). These target viruses were exposed to AgNP-MHCs for 1, 3, and 6 h at 25 degrees C and then analyzed by plaque assay and real-time TaqMan PCR. The AgNP-MHCs were exposed to a wide range of pH levels and to tap and surface water to assess their antiviral effects under different environmental conditions. Among the three types of AgNP-MHCs tested, Ag30-MHCs displayed the highest efficacy for inactivating the viruses. The phi X174 and MNV were reduced by more than 2 log(10) after exposure to 4.6 x 10(9) Ag30-MHCs/ml for 1 h. These results indicated that the AgNP-MHCs could be used to inactivate viral pathogens with minimum chance of potential release into environment. English AMER SOC MICROBIOLOGY MURINE NOROVIRUS ANTIBACTERIAL ACTIVITY CYTOTOXICITY DISINFECTION INACTIVATION VIRUS MODEL ADENOVIRUSES NANOSILVER GOLD Antiviral Properties of Silver Nanoparticles on a Magnetic Hybrid Colloid Article 10.1128/AEM.03427-13 1 APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.80, no.8, pp.2343 - 2350 APPLIED AND ENVIRONMENTAL MICROBIOLOGY 80 8 2343 2350 scie scopus 000333531600003 2-s2.0-84896901463 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article MURINE NOROVIRUS ANTIBACTERIAL ACTIVITY CYTOTOXICITY DISINFECTION INACTIVATION VIRUS MODEL ADENOVIRUSES NANOSILVER GOLD Antiviral silver nanoparticle magnetic hybrid colloid pathogen environment bacteriophage