Reinauer, Kimberly M. Popovic, Jovan Weber, Christopher D. Millerick, Kayleigh A. Kwon, Man Jae Wei, Na Zhang, Yang Finneran, Kevin T. 2024-01-20T10:02:55Z 2024-01-20T10:02:55Z 2021-09-05 2014-04 0343-8651 https://pubs.kist.re.kr/handle/201004/126937 A Gram-negative, rod-shaped bacterium was isolated from a mixed culture that degraded tert-butyl alcohol (TBA) in a granular-activated carbon (GAC) sample from a Biological-GAC reactor. Strain YZ2(T) was assigned to the Betaproteobacteria within the family Comamonadaceae based on 16S rRNA gene similarities. The nearest phylogenetic relative (95.0 % similarity) with a valid name was Hydrogenophaga taeniospiralis. The DNA G+C content was 66.4 mol%. DNA:DNA hybridization indicated that the level of relatedness to members of the genus Hydrogenophaga ranged from 1.1 to 10.8 %. The dominant cellular fatty acids were: 18:1 w7c (75 %), 16:0 (4.9 %), 17:0 (3.85 %), 18:0 (2.93 %), 11 methyl 18:1 w7c (2.69 %), Summed Feature 2 (2.27 %), and 18:0 3OH (1.35 %). The primary substrate used was TBA, which is a fuel oxygenate and groundwater contaminant. YZ2(T) was non-motile, without apparent flagella. It is a psychrotolerant, facultative aerobe that grew between pH 6.5 and 9.5, and 4 and 30 A degrees C. The culture grew on and mineralized TBA at 4 A degrees C, which is the first report of psychrotolerant TBA degradation. Hydrogen was used as an alternative electron donor. The culture also grew well in defined freshwater medium with ethanol, butanol, hydroxy isobutyric acid, acetate, pyruvate, citrate, lactate, isopropanol, and benzoic acid as electron donors. Nitrate was reduced with hydrogen as the sole electron donor. On the basis of morphological, physiological, and chemotaxonomic data, a new species, Hydrogenophaga carboriunda is proposed, with YZ2(T) as the type strain. English SPRINGER LOW-TEMPERATURE POLYCHLORINATED-BIPHENYLS GEN. NOV. BIODEGRADATION ETHER BACTERIA MTBE DEGRADATION STRAIN FLAVA Hydrogenophaga carboriunda sp nov., a Tertiary Butyl Alcohol-Oxidizing, Psychrotolerant Aerobe Derived from Granular-Activated Carbon (GAC) Article 10.1007/s00284-013-0501-8 1 CURRENT MICROBIOLOGY, v.68, no.4, pp.510 - 517 CURRENT MICROBIOLOGY 68 4 510 517 scie scopus 000331006200013 2-s2.0-84893930272 Microbiology Microbiology Article LOW-TEMPERATURE POLYCHLORINATED-BIPHENYLS GEN. NOV. BIODEGRADATION ETHER BACTERIA MTBE DEGRADATION STRAIN FLAVA