Lee, Jun-Seok Yoo, Young-Hwa Yoon, Chang No 2024-01-20T10:03:43Z 2024-01-20T10:03:43Z 2021-09-04 2014-03-31 1976-6696 https://pubs.kist.re.kr/handle/201004/126977 The recent dramatic improvements in high-resolution mass spectrometry (MS) have revolutionized the speed and scope of proteomic studies. Conventional MS-based proteomics methodologies allow global protein profiling based on expression levels. Although these techniques are promising, there are numerous biological activities yet to be unveiled, such as the dynamic regulation of enzyme activity. Chemical proteomics is an emerging field that extends these types proteomic profiling. In particular, activity-based protein profiling (ABPP) utilizes small-molecule probes to monitor enzyme activity directly in living intact subjects. In this mini-review, we summarize the unique roles of small-molecule probes in proteomics studies and highlight some recent examples in which this principle has been applied. English KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY SPECTROMETRY-BASED PROTEOMICS MASS-SPECTROMETRY QUANTITATIVE PROTEOMICS GEL-ELECTROPHORESIS HUMAN GENOME PROTEIN IDENTIFICATION INHIBITORS DISCOVERY CHEMISTRY Small-molecule probes elucidate global enzyme activity in a proteomic context Article 10.5483/BMBRep.2014.47.3.264 1 BMB REPORTS, v.47, no.3, pp.149 - 157 BMB REPORTS 47 3 149 157 scie scopus kci ART001859830 000333672500006 2-s2.0-84896950653 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Review SPECTROMETRY-BASED PROTEOMICS MASS-SPECTROMETRY QUANTITATIVE PROTEOMICS GEL-ELECTROPHORESIS HUMAN GENOME PROTEIN IDENTIFICATION INHIBITORS DISCOVERY CHEMISTRY Activity-based protein profiling Fluorescent imaging Proteomics Small molecule probes Systems biology