Kang, Kyungsu Song, Dae-Geun Lee, Eun Ha Lee, Kyung-Mi Park, Young Gyun Jung, Sang Hoon Pan, Cheol-Ho Nho, Chu Won 2024-01-20T10:03:54Z 2024-01-20T10:03:54Z 2021-09-05 2014-03-19 0021-8561 https://pubs.kist.re.kr/handle/201004/126986 Dietary polyacetylenes from various foods have been receiving attention as promising cancer chemopreventive agents. However, until now, the detailed molecular mechanism and the regulatory proteins underlying these effects have not been elucidated. We investigated the effects of gymnasterkoreayne B (GKB), a model dietary polyacetylene from wild vegetables, on the programmed cell death of HCT116 human colorectal cancer cells. GKB inhibited HCT116 cell proliferation by inducing apoptotic cell death. GKB treatment resulted in ROS accumulation, leading to the activation of both intrinsic and extrinsic apoptotic pathway. We also found that FN1, TGFBI, APP, SERPINE1, HSPD1, SOD1, TXN, and ACTN4 may act as secretory signaling molecules during GKB-induced apoptotic cell death using LC-MS/MS identification followed by spectrum counting, statistical calculation, and gene ontology analysis. The secretory proteins suggested in this study may be promising candidates involved in apoptotic cell death of cancer cells induced by GKB that warrant further functional study. English AMER CHEMICAL SOC COLON-CANCER PROTEINS INDUCTION ACTIVATION DEATH COMPONENTS EXPRESSION VEGETABLES INHIBITOR CASPASE-3 Secretome Profiling Reveals the Signaling Molecules of Apoptotic HCT116 Cells Induced by the Dietary Polyacetylene Gymnasterkoreayne B Article 10.1021/jf404047z 1 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.62, no.11, pp.2353 - 2363 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62 11 2353 2363 scie scopus 000333435700005 2-s2.0-84896516844 Agriculture, Multidisciplinary Chemistry, Applied Food Science & Technology Agriculture Chemistry Food Science & Technology Article COLON-CANCER PROTEINS INDUCTION ACTIVATION DEATH COMPONENTS EXPRESSION VEGETABLES INHIBITOR CASPASE-3 gymnasterkoreayne B polyacetylene programmed cell death secretome cancer