Lee, Sangmin Kang, Sun-Woong Ryu, Ju Hee Na, Jin Hee Lee, Dong-Eun Han, Seung Jin Kang, Choong Mo Choe, Yearn Seong Lee, Kyo Chul Leary, James F. Choi, Kuiwon Lee, Kyung-Han Kim, Kwangmeyung 2024-01-20T10:04:45Z 2024-01-20T10:04:45Z 2021-09-05 2014-03 1043-1802 https://pubs.kist.re.kr/handle/201004/127029 Imaging techniques including computed tomography, magnetic resonance imaging, and positron emission tomography (PET) offer many potential benefits to diagnosis and treatment of cancers. Each method has its own strong and weak points. Therefore, multimodal imaging techniques have been highlighted as an alternative method for overcoming the limitations of each respective imaging method. In this study, we fabricated PET/optical activatable imaging probe based on glycol chitosan nanoparticles (GNPs) for multimodal imaging. To prepare the dual PET/optical probes based on CNPs, both Cu-64 radiolabeled DOTA complex and activatable matrix metalloproteinase (MMP)-sensitive peptide were chemically conjugated onto azide-functionalized CNPs via bio-orthogonal click chemistry, which was a reaction between azide group and dibenzyl cyclooctyne. The PET/optical activatable imaging probes were visualized by PET and optical imaging system. Biodistribution of probes and activity of MNIP were successfully measured in tumor-bearing mice. English AMER CHEMICAL SOC FREE CLICK CHEMISTRY MATRIX METALLOPROTEINASES IN-VIVO MULTIFUNCTIONAL NANOPARTICLES CATHEPSIN-B REAL-TIME DELIVERY THERAPY PARTICLES MICROPET Tumor-Homing Glycol Chitosan-Based Optical/PET Dual Imaging Nanoprobe for Cancer Diagnosis Article 10.1021/bc500020g 1 BIOCONJUGATE CHEMISTRY, v.25, no.3, pp.601 - 610 BIOCONJUGATE CHEMISTRY 25 3 601 610 scie scopus 000333435900016 2-s2.0-84896524686 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Multidisciplinary Chemistry, Organic Biochemistry & Molecular Biology Chemistry Article FREE CLICK CHEMISTRY MATRIX METALLOPROTEINASES IN-VIVO MULTIFUNCTIONAL NANOPARTICLES CATHEPSIN-B REAL-TIME DELIVERY THERAPY PARTICLES MICROPET