Choi, Kee-Hyun 2024-01-20T10:04:48Z 2024-01-20T10:04:48Z 2021-09-05 2014-03 0231-5882 https://pubs.kist.re.kr/handle/201004/127032 Cooperative gating between ion channels, i.e. the gating of one channel directly coupled to the gating of neighboring channels, has been observed in diverse channel types at the single-channel level. Positively coupled gating could enhance channel-mediated signaling while negative coupling may effectively reduce channel gating noise. Indeed, the physiological significance of cooperative channel gating in signal transduction has been recognized in several in vivo studies. Moreover, coupled gating of ion channels was reported to be associated with some human disease states. In this review, physiological roles for channel cooperativity and channel clustering observed in vitro and in vivo are introduced, and stimulation-induced channel clustering and direct channel cross linking are suggested as the physical mechanisms of channel assembly. Along with physical clustering, several molecular mechanisms proposed as the molecular basis for functional coupling of neighboring channels are covered: permeant ions as a channel coupling mediator, concerted channel activation through the membrane, and allosteric mechanisms. Also, single-channel analysis methods for cooperative gating such as the binomial analysis, the variance analysis, the conditional dwell time density analysis, and the maximum likelihood fitting analysis are reviewed and discussed. English GENERAL PHYSIOL AND BIOPHYSICS SUPERPOSITION PROPERTIES ACETYLCHOLINE-RECEPTORS RELEASE CHANNEL CA2+ SKELETAL NOISE MEMBRANE CONDUCTANCE PRECISION MECHANOSENSITIVITY Cooperative gating between ion channels Article 10.4149/gpb_2013076 1 GENERAL PHYSIOLOGY AND BIOPHYSICS, v.33, no.1, pp.1 - 12 GENERAL PHYSIOLOGY AND BIOPHYSICS 33 1 1 12 scie scopus 000332189800001 2-s2.0-84894112851 Biochemistry & Molecular Biology Biophysics Physiology Biochemistry & Molecular Biology Biophysics Physiology Article SUPERPOSITION PROPERTIES ACETYLCHOLINE-RECEPTORS RELEASE CHANNEL CA2+ SKELETAL NOISE MEMBRANE CONDUCTANCE PRECISION MECHANOSENSITIVITY Ion channel Cooperative gating Functional coupling Single-channel analysis