Kim, J. Park, J. H. Lee, S. Shin, H. J. 2024-01-20T10:30:22Z 2024-01-20T10:30:22Z 2021-09-05 2014-03 1533-4880 https://pubs.kist.re.kr/handle/201004/127054 In this paper, we introduce a simple and easy DNA mapping method using peptide nucleic acid (PNA) on glass cover-slips based on optical detection. PNA and glass cover-slips were used as easy tagging and stretching method, separately. The PNA can be invaded lambda (lambda) DNA at the sequences we wished to tag without any additional materials. Alexa-488 fluorophore-conjugated PNA was designed to invade at four sites (sequence: AAGAAGAA) of lambda DNA with robust and exact binding. Fluorophore tagged lambda DNA was stretched in electrical layer coated glass cover-slips. The lambda DNA was stretched as 9.2 um in length. It is approximately 60% of theoretical length of lambda DNA and sufficient length to promise optical resolution. The sites of desired sequence tagged by PNA were well detected in stretched lambda DNA. English AMER SCIENTIFIC PUBLISHERS SINGLE-MOLECULE DETECTION GEL-ELECTROPHORESIS Simple and Easy DNA Mapping Method Using Peptide Nucleic Acid (PNA) Tagging Article 10.1166/jnn.2014.8494 1 JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.14, no.3, pp.2477 - 2481 JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 14 3 2477 2481 scie scopus 000331624700047 2-s2.0-84894140942 Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article SINGLE-MOLECULE DETECTION GEL-ELECTROPHORESIS DNA Sequencing DNA Mapping DNA Stretching PNA Single Molecule Detection.