Kim, Miok Song, Na Rae Choi, Jong-Ho Lee, Jeongae Pyo, Heesoo 2024-01-20T10:31:39Z 2024-01-20T10:31:39Z 2021-09-04 2014-02-01 0048-9697 https://pubs.kist.re.kr/handle/201004/127117 Phthalates are used in industry products, household items, and medical tools as plasticizers. Human exposure to phthalates has raised concern about its toxicity. In the present study, optimization was conducted for the simultaneous analysis of eight kinds of phthalate metabolites using gas chromatography-mass spectrometry (GC-MS): MEP, MiBP, MnBP, MBzP, MiNP, MEHP, MEOHP, and MEHHP. In order to minimize the matrix effect and to do quantitative analysis, isotope dilution and LLE-GC-MS methods were performed. Urine samples were enzymatically hydrolyzed, extracted with a mixture of n-hexane and ethyl ether (8:2; v:v), and subsequently derivatized with trimethylsilylation. All eight kinds of analytes showed clear resolution and high reproducibility in GC-MS results. The method detection limit ranged from 0.05 ng/mL to 0.2 ng/mL. Calibration curves were found to be linear from 0.2 to 100 ng/mL with -(2) > 0.992. The relative standard deviation of the intraday precision using water and urine ranged from 2.1% to 163%. The analysis was performed with urine samples that were collected from adults residing in the Republic of Korea. The analyzed concentration results were compared according to gender and region. As a result, DEHP metabolites showed the highest detected concentration (75.92 mu g/g creatinine, 100%), and MiNP, a metabolite of DiNP, showed the lowest detected concentration (0.42 mu g/g creatinine, 22.5%). On average, female urine (200.76 mu g/g creatinine) had a higher detected concentration of Sigma(8) phthalate metabolites than male urine. Samples from rural regions (211.96 mu g/g creatinine) had higher levels than samples from urban regions. (C) 2013 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV SOLID-PHASE EXTRACTION DI(2-ETHYLHEXYL) PHTHALATE GENERAL-POPULATION CHILD-PAIRS DEHP EXPOSURE DI-(2-ETHYLHEXYL)PHTHALATE PHARMACOKINETICS MOTHER RATS Simultaneous analysis of urinary phthalate metabolites of residents in Korea using isotope dilution gas chromatography-mass spectrometry Article 10.1016/j.scitotenv.2013.07.037 1 SCIENCE OF THE TOTAL ENVIRONMENT, v.470, pp.1408 - 1413 SCIENCE OF THE TOTAL ENVIRONMENT 470 1408 1413 scie scopus 000331415600147 2-s2.0-84891741624 Environmental Sciences Environmental Sciences & Ecology Article; Proceedings Paper SOLID-PHASE EXTRACTION DI(2-ETHYLHEXYL) PHTHALATE GENERAL-POPULATION CHILD-PAIRS DEHP EXPOSURE DI-(2-ETHYLHEXYL)PHTHALATE PHARMACOKINETICS MOTHER RATS Urinary phthalate metabolites Gas chromatography-mass spectrometry Trimethylsilylation Isotope dilution Biomonitoring