Simone, Giuseppina Malara, Natalia Trunzo, Valentina Renne, Maria Perozziello, Gerardo Di Fabrizio, Enzo Manz, Andreas 2024-01-20T10:31:50Z 2024-01-20T10:31:50Z 2022-01-10 2014-02 1742-206X https://pubs.kist.re.kr/handle/201004/127127 Galectin-3, beta-galactoside-binding lectin, coats the membrane of most cancer cells and is involved in metastasis and endothelium recognition as well as in evading immune surveillance through killing of activated T cells. To flag galectin as a biomarker of tumours and metastasis, it is pivotal to understand the role of this protein in different tumours and at different stages. Breast tumours have an anomalous behaviour of the galectin-3 compared to other tumour cells. Herein, FACS sorting and galactoside based assays were used to investigate the role of galectin-3 in metastasis and metastatisation of breast cancer cells. Breast galectin fingerprint at the FACS displayed a higher amount in healthy cells, compared to metastatic cells. The microfluidic assay was able to isolate tumour and metastatic cells more than healthy breast cells. Investigation was performed on samples from patients with breast tumours at stage I and stage III whilst MCF7 and EPH-4 cells were used to perform preliminary investigations. The readout of the conditioned medium (from culturing of stage I cells) fingerprint by FACS evidenced high expression of free galectin. Analysis of the results established that the galectin coating the membrane, by galactoside recognition of the breast cells, and engaged by the cells to form protein-carbohydrate complexes inside the microfluidic assay, resembled the tumour signature of tumours in breast cells whilst the galectin free is independent of those mechanisms. English ROYAL SOC CHEMISTRY CANCER METASTASIS GLYCOSYLATION MICROARRAY EXPRESSION MODULATORS CARCINOMA Galectin-3 coats the membrane of breast cells and makes a signature of tumours Article 10.1039/c3mb70359b 1 MOLECULAR BIOSYSTEMS, v.10, no.2, pp.258 - 265 MOLECULAR BIOSYSTEMS 10 2 258 265 scie scopus 000328953000011 2-s2.0-84891378348 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article CANCER METASTASIS GLYCOSYLATION MICROARRAY EXPRESSION MODULATORS CARCINOMA