권만재 양중석 심무준 이승학 Maxim Boyanov Kenneth Kemner Edward O'Loughlin 2024-01-20T10:31:54Z 2024-01-20T10:31:54Z 2021-09-06 2014-02 1598-6438 https://pubs.kist.re.kr/handle/201004/127130 To better understand dissimilatory iron and sulfate reduction (DIR and DSR) by subsurface microorganisms, we investigated the effects of sulfate and electron donors on the microbial goethite (α-FeOOH) reduction. Batch systems were created 1) with acetate or glucose (donor), 2) with goethite and sulfate (acceptor), and 3) with aquifer sediment (microbial source). With 0.2 mM sulfate, goethite reduction coupled with acetate oxidation was limited. However, with 10 mM sulfate, 8 mM goethite reduction occurred with complete sulfate reduction and x-ray absorption fine-structure analysis indicated the formation of iron sulfide. This suggests that goethite reduction was due to the sulfide species produced by DSR bacteria rather than direct microbial reaction by DIR bacteria. Both acetate and glucose promoted goethite reduction. The rate of goethite reduction was faster with glucose, while the extent of goethite reduction was higher with acetate. Sulfate reduction (10 mM) occurred only with acetate. The results suggest that glucose-fermenting bacteria rapidly stimulated goethite reduction, but acetate-oxidizing DSR bacteria reduced goethite indirectly by producing sulfides. This study suggests that the availability of specific electron donor and sulfate significantly influence microbial community activities as well as goethite transformation, which should be considered for the bioremediation of contaminated environments. Korean 한국지하수토양환경학회 전자공여체와 황산염 이용 토착미생물에 의한 침철석(α-FeOOH) 환원 연구 Biotic and Abiotic Reduction of Goethite (α-FeOOH) by Subsurface Microorganisms in the Presence of Electron Donor and Sulfate Article 2 지하수토양환경, v.19, no.1, pp.54 - 62 지하수토양환경 19 1 54 62 kci ART001853800 Goethite Acetate Glucose Sulfate Microorganisms