Ryu, Ju Hee Na, Jin Hee Ko, Ho Kyung You, Dong Gil Park, Subin Jun, Eunsung Yeom, Ho Jun Seo, Deok Ho Park, Jae Hyung Jeong, Seo Young Kim, In-San Kim, Byung-Soo Kwon, Ick Chan Choi, Kuiwon Kim, Kwangmeyung 2024-01-20T10:32:01Z 2024-01-20T10:32:01Z 2021-09-05 2014-02 0142-9612 https://pubs.kist.re.kr/handle/201004/127136 An increasing number of treatments of metastases rely on diagnostics and imaging these days. The facts that the activity of cathepsin B (CB) is markedly linked to the metastatic process and that CB is found highly expressed in the pericellular regions in this process make CB an attractive target for diagnosing metastases. We have developed a CB-sensitive nanoprobe (CB-CNP) consisting of self-quenched CB-sensitive fluorogenic peptide probes conjugated onto the surface of tumor-targeting glycol chitosan nanoparticles (CNPs). The freshly prepared CB-CNP formed a spherical nanoparticle structure (280 nm in diameter) and the fluorescence intensity of CB-CNP was strongly quenched in physiological condition. However, self-quenched CB-CNP boosted strong fluorescence signals in the presence of CB, not of cathepsin I. or cathepsin D, due to the CB-specific cleavage of self-quenched peptide probes. Importantly, the intravenously injected CB-CNP demonstrated the potential to discriminate metastases in vivo in three metastatic mouse models, including 4T1-luc2 liver metastases, RFP-B16F10 lung metastases and HT1080 peritoneal metastases. Indeed, Western blot analysis confirmed that the CB expression of metastases had increased compared to normal organ in these metastatic mouse models. CB-CNPs may be useful for depicting metastases through non-invasive CB molecular imaging. (C) 2013 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD GLYCOL CHITOSAN NANOPARTICLES CARCINOMA-CELLS REAL-TIME TUMOR CANCER PERITONEAL GROWTH INHIBITION EXPRESSION SELECTION Non-invasive optical imaging of cathepsin B with activatable fluorogenic nanoprobes in various metastatic models Article 10.1016/j.biomaterials.2013.11.080 1 BIOMATERIALS, v.35, no.7, pp.2302 - 2311 BIOMATERIALS 35 7 2302 2311 scie scopus 000331502300024 2-s2.0-84891372016 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article GLYCOL CHITOSAN NANOPARTICLES CARCINOMA-CELLS REAL-TIME TUMOR CANCER PERITONEAL GROWTH INHIBITION EXPRESSION SELECTION Cathepsin B Metastasis Molecular imaging Metastatic mouse model Fluorescence