Hwang, Yu Jin Han, Dohyun Kim, Ki Yoon Min, Sun-Joon Kowall, Neil W. Yang, Liu Lee, Junghee Kim, Youngsoo Ryu, Hoon 2024-01-20T10:32:27Z 2024-01-20T10:32:27Z 2021-09-05 2014-02 0305-1048 https://pubs.kist.re.kr/handle/201004/127159 The remodeling of chromatin in the nucleolus is important for the control of ribosomal DNA (rDNA) transcription and ribosome biogenesis. Herein, we found that upstream binding factor (UBF) interacts with ESET, a histone H3K9 methyltransferase and is trimethylated at Lys (K) 232/254 by ESET. UBF trimethylation leads to nucleolar chromatin condensation and decreased rDNA transcriptional activity. UBF mutations at K232/254A and K232/254R restored rDNA transcriptional activity in response to ESET. Both ESET-Delta SET mutant and knockdown of ESET by short hairpin RNA reduced trimethylation of UBF and resulted in the restoration of rDNA transcription. Atomic force microscopy confirmed that UBF trimethylated by ESET modulates the plasticity of nucleolar chromatin. We further demonstrated that UBF trimethylation at K232/254 by ESET deregulates rDNA transcription in a cell model of Huntington's disease. Together, our findings show that a novel epigenetic modification of UBF is linked to impaired rDNA transcription and nucleolar chromatin remodeling, which may play key roles in the pathogenesis of neurodegeneration. English OXFORD UNIV PRESS RIBOSOMAL-RNA GENES HISTONE H3 BINDING PROTEIN CBP POLYGLUTAMINE ACETYLATION EXPRESSION METHYLTRANSFERASE CONDENSATION HUNTINGTIN ESET methylates UBF at K232/254 and regulates nucleolar heterochromatin plasticity and rDNA transcription Article 10.1093/nar/gkt1041 1 NUCLEIC ACIDS RESEARCH, v.42, no.3, pp.1628 - 1643 NUCLEIC ACIDS RESEARCH 42 3 1628 1643 scie scopus 000331138800024 2-s2.0-84896701163 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article RIBOSOMAL-RNA GENES HISTONE H3 BINDING PROTEIN CBP POLYGLUTAMINE ACETYLATION EXPRESSION METHYLTRANSFERASE CONDENSATION HUNTINGTIN