Feng, Min Ibrahim, Basma M. Wilson, Erin M. Doh, Kyung-Oh Bergman, Brandon K. Park, Christopher Yeo, Yoon 2024-01-20T10:32:38Z 2024-01-20T10:32:38Z 2021-09-05 2014-02 2047-4830 https://pubs.kist.re.kr/handle/201004/127169 A "DPH" ternary complex consisting of plasmid DNA (pDNA), intracellularly degradable polyethyleneimine, and hyaluronic acid (HA) is a promising non-viral gene carrier with low toxicity and good gene transfection efficiency. HA plays a key role in providing an optimal balance between DNA protection and release, but it causes aggregation due to the entanglement of HA chains of neighbouring DPH particles. Here we report that the addition of an optimal level of Ca2+ successfully prevents particle aggregation and maintains a relatively small size. The Ca-stabilized DPH is comparable to DPH in cytotoxicity and gene transfection efficiency. MW monitoring and conductometric titration suggest that such size stabilization effect is partly mediated by the complexation between HA and Ca2+, which enables intra-and intermolecular interactions of HAs. English ROYAL SOC CHEMISTRY PLASMID-LIPID PARTICLES EGG-BOX MODEL DIVALENT-CATIONS MAMMALIAN-CELLS SIRNA DELIVERY TRANSFECTION ACID CONFORMATION COMPLEXES GELATION Stabilization of a hyaluronate-associated gene delivery system using calcium ions Article 10.1039/c4bm00012a 1 BIOMATERIALS SCIENCE, v.2, no.6, pp.936 - 942 BIOMATERIALS SCIENCE 2 6 936 942 scie scopus 000335984000014 2-s2.0-84900332247 Materials Science, Biomaterials Materials Science Article PLASMID-LIPID PARTICLES EGG-BOX MODEL DIVALENT-CATIONS MAMMALIAN-CELLS SIRNA DELIVERY TRANSFECTION ACID CONFORMATION COMPLEXES GELATION