Park, Se Jin Schertel, Andreas Lee, Kyung Eun Han, Sung Sik 2024-01-20T10:32:54Z 2024-01-20T10:32:54Z 2021-09-04 2014-02 2050-5698 https://pubs.kist.re.kr/handle/201004/127184 Alzheimer's disease (AD), one of the most prevalent neurodegenerative brain diseases, has been extensively researched for years. However, its synaptic structure, which is a basis for understanding neurodegenerative disorders, has not yet been understood clearly. Defining the structures of neurons and their synaptic connections is the significant goal of brain research. To study synaptic connectivity, three-dimensional (3D) reconstructions of the nervous system are very helpful. In this study, the 3D structure of brain synapses in the Drosophila melanogaster Swedish amyloid precursor protein (APP) mutant, which is characterized by early onset AD, was analyzed using focused ion beam/scanning electron microscopy (FIB/SEM). This technique is one of the most useful for 3D reconstruction, as the process of obtaining serial images is fully automated and thus avoids the problems inherent in hand-operated ultrathin serial sectioning. The 3D images of normal and AD brains reported in this study reveal characteristic features of AD such as appearance of autophagy, abnormal axon formation and increased mitochondrial size. This 3D analysis reveals structural change as a basis for understanding neurodegenerative disorder. English OXFORD UNIV PRESS PRECURSOR PROTEIN APP MITOCHONDRIAL FISSION AMYLOID HYPOTHESIS AUTOPHAGY FUSION SYNAPTOTAGMIN SYNAPSES BALANCE CELLS SIZE Ultra-structural analysis of the brain in a Drosophila model of Alzheimer's disease using FIB/SEM microscopy Article 10.1093/jmicro/dft039 1 MICROSCOPY, v.63, no.1, pp.3 - 13 MICROSCOPY 63 1 3 13 scopus 000331875600002 2-s2.0-84907391053 Microscopy Microscopy Article PRECURSOR PROTEIN APP MITOCHONDRIAL FISSION AMYLOID HYPOTHESIS AUTOPHAGY FUSION SYNAPTOTAGMIN SYNAPSES BALANCE CELLS SIZE FIB/SEM 3D structure Alzheimer&apos s disease axon terminal mitochondria