Seo, Cho-Rong Yang, Dong Kwon Song, No-Joon Yun, Ui Jeong Gwon, A-Ryeong Jo, Dong-Gyu Cho, Jae Youl Yoon, Keejung Ahn, Jee-Yin Nho, Chu Won Park, Woo Jin Yang, Seung Yul Park, Kye Won 2024-01-20T10:32:55Z 2024-01-20T10:32:55Z 2021-09-04 2014-02 0278-6915 https://pubs.kist.re.kr/handle/201004/127185 Cucurbitacin B, a member of the cucurbitaceae family, can act as a STAT3 signaling inhibitor to regulate the growth of hepatocellular carcinoma. STAT3 signaling has been shown to inhibit adipocyte differentiation through C/EBP alpha and PPAR gamma. Based on these studies, we hypothesized that cucurbitacin B would prevent PPAR gamma mediated adipocyte differentiation through STAT3 signaling. To test this hypothesis, mesenchymal C3H10T1/2 and 3T3-L1 preadipocyte cells were treated with a sub-cytotoxic concentration of cucurbitacin B. Cucurbitacin B treatment inhibits lipid accumulation and expression of adipocyte markers including PPAR gamma and its target genes in a dose-dependent manner. Cucurbitacin B treatment impairs STAT3 signaling as manifested by reduced phosphorylation of STAT3 and suppression of STAT3 target gene expression in preadipocytes. The anti-adipogenic effects of cucurbitacin B are significantly blunted in cells with STAT3 silenced by introducing small interfering RNA. Finally, our data show that cucurbitacin I, another cucurbitacin family member, also inhibits adipocyte differentiation by suppressing STAT3 signaling. Together, our data suggest the possibility of utilizing cucurbitacins as a new strategy to treat metabolic diseases and implicate STAT3 as a new target for the development of functional foods and drugs. (C) 2013 Elsevier Ltd. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD PANCREATIC-CANCER CELLS EPITHELIAL-CELLS OBESITY DIET ACTIVATION APOPTOSIS COMPOUND PATHWAY Cucurbitacin B and cucurbitacin I suppress adipocyte differentiation through inhibition of STAT3 signaling Article 10.1016/j.fct.2013.11.040 1 FOOD AND CHEMICAL TOXICOLOGY, v.64, pp.217 - 224 FOOD AND CHEMICAL TOXICOLOGY 64 217 224 scie scopus 000331419100026 2-s2.0-84890647788 Food Science & Technology Toxicology Food Science & Technology Toxicology Article PANCREATIC-CANCER CELLS EPITHELIAL-CELLS OBESITY DIET ACTIVATION APOPTOSIS COMPOUND PATHWAY Cucurbitacin B Adipocyte differentiation STAT3 PPAR gamma Cucurbitacin I