You, Dong Gil Sarayanakumar, Gurusamy Son, Soyoung Han, Hwa Seung Heo, Roun Kim, Kwangmeyung Kwon, Ick Chan Lee, Jun Young Park, Jae Hyung 2024-01-20T10:33:18Z 2024-01-20T10:33:18Z 2021-09-05 2014-01-30 0144-8617 https://pubs.kist.re.kr/handle/201004/127202 The hallmark of atherosclerosis in its early pathogenic process is the overexpression of class A scavenger receptors (SR-A) by activated macrophages. In this study, dextran sulfate-coated superparamagnetic iron oxide nanoparticles (DS-SPIONs), as a magnetic resonance (MR) imaging contrast agent of atherosclerosis, was prepared via the facile co-precipitation method using a versatile double-hydrophilic block copolymer comprising of a DS segment (ligand for SR-A) and a poly(glyclerol methacrylate) segment (SPIONs surface-anchoring unit). The physicochemical properties of the DS-SPIONs were investigated using various instruments. DS-SPIONs exhibited high aqueous stability compared to dextran-coated SPIONs (Dex-SPIONs), which were used as controls. The cellular uptake behaviors of DS-SPIONs and Dex-SPIONs were evaluated using Prussian blue assay. Interestingly, the DS-SPIONs were effectively taken up by activated macrophages compared to Dex-SPION5. However, the cellular uptake of DS-SPIONs by activated macrophages was remarkably reduced in the presence of free DS. These results suggest that activated macrophages internalize DS-SPIONs via receptor (SR-A)-mediated endocytosis. T-2-weighted MR imaging of the cells demonstrated that activated macrophages treated with DS-SPIONs showed a significantly lower signal intensity compared to those treated with Dex-SPIONs. Overall, these results suggest that DS-SPIONs may be utilized as a potential contrast agent for atherosclerosis MR imaging. (C) 2013 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD IN-VIVO DETECTION MAGNETIC-RESONANCE SCAVENGER RECEPTOR CLASS-A MACROPHAGES PARTICLES CANCER PLAQUE INFLAMMATION MECHANISMS Dextran sulfate-coated superparamagnetic iron oxide nanoparticles as a contrast agent for atherosclerosis imaging Article 10.1016/j.carbpol.2013.10.068 1 CARBOHYDRATE POLYMERS, v.101, pp.1225 - 1233 CARBOHYDRATE POLYMERS 101 1225 1233 scie scopus 000330494800154 2-s2.0-84887338966 Chemistry, Applied Chemistry, Organic Polymer Science Chemistry Polymer Science Article IN-VIVO DETECTION MAGNETIC-RESONANCE SCAVENGER RECEPTOR CLASS-A MACROPHAGES PARTICLES CANCER PLAQUE INFLAMMATION MECHANISMS Dextran sulfate Atherosclerosis Double hydrophilic copolymer Contrast agent Magnetic resonance imaging