Jang, Holim Ahn, Hong Ryul Jo, Hyoung Kim, Kyung-A Lee, Eun Ha Lee, Ki Won Jung, Sang Hoon Lee, Chang Y. 2024-01-20T10:33:48Z 2024-01-20T10:33:48Z 2021-09-05 2014-01-08 0021-8561 https://pubs.kist.re.kr/handle/201004/127226 This study explored whether chlorogenic acid (CGA) and coffee have protective effects against retinal degeneration. Under hypoxic conditions, the viability of transformed retinal ganglion (RGC-5) cells was significantly reduced by treatment with the nitric oxide (NO) donor S-nitroso-N-acetylpenicillamine (SNAP). However, pretreatment with CGA attenuated cell death in a concentration-dependent manner. In addition, CGA prevented the up-regulation of apoptotic proteins such as Bad and cleaved caspase-3. Similar beneficial effects of both CGA and coffee extracts were observed in mice that had undergone an optic nerve crush (ONC) procedure. CGA and coffee extract reduced cell death by preventing the down-regulation of Thy-1. Our in vitro and in vivo studies demonstrated that coffee and its major component, CGA, significantly reduce apoptosis of retinal cells induced by hypoxia and NO, and that coffee consumption may help in preventing retinal degeneration. English AMER CHEMICAL SOC APOPTOTIC CELL-DEATH OPTIC-NERVE CRUSH GANGLION-CELLS IN-VITRO CAFFEINATED COFFEE CEREBRAL-ISCHEMIA NITRIC-OXIDE RGC-5 CELLS CONSUMPTION ANTIOXIDANT Chlorogenic Acid and Coffee Prevent Hypoxia-Induced Retinal Degeneration Article 10.1021/jf404285v 1 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.62, no.1, pp.182 - 191 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62 1 182 191 scie scopus 000329586800023 2-s2.0-84892182545 Agriculture, Multidisciplinary Chemistry, Applied Food Science & Technology Agriculture Chemistry Food Science & Technology Article APOPTOTIC CELL-DEATH OPTIC-NERVE CRUSH GANGLION-CELLS IN-VITRO CAFFEINATED COFFEE CEREBRAL-ISCHEMIA NITRIC-OXIDE RGC-5 CELLS CONSUMPTION ANTIOXIDANT coffee chlorogenic acid neuroprotection retinal degeneration hypoxia RGC-5 glaucoma