Kim, Da-Rae Kim, Taek-Soo Kim, Eunsun Min, Sun-Joon Shin, Dongyun Ahn, Dae-Ro 2024-01-20T10:34:04Z 2024-01-20T10:34:04Z 2021-09-05 2014-01-01 0960-894X https://pubs.kist.re.kr/handle/201004/127239 Next-generation sequencing (NGS) technologies recently developed are now used for study of genomes from various organisms. Sequencing-by-synthesis (SBS) is a key strategy in the NGS. The SBS uses nucleotides so-called dual-modified reversible terminators (DRTs) in which bases are labeled with fluorophores and 3'-OH is protected with a reversibly cleavable chemical group, respectively. In this study, we examined the possibility of performing SBS with mono-modified reversible terminators (MRTs), in which the reversible blocking group on the 3'-OH plays a dual role as a fluorescent signal report as well as a chemical protection. We studied cyclic reversible termination by using two MRTs (dA and dT), wherein the modifications were two different fluorophores and cleavable to regenerate a free 3'-OH. We here demonstrated that SBS could be achieved with incorporation of MRTs by a DNA polymerase and correct base-calls based on the two different colors from the fluorophores. (C) 2013 Elsevier Ltd. All rights reserved. English Pergamon Press Ltd. DNA-POLYMERASE TRIPHOSPHATES NUCLEOTIDES Synthesis of 3 '-O-fluorescently mono-modified reversible terminators and their uses in sequencing-by-synthesis Article 10.1016/j.bmcl.2013.11.040 1 Bioorganic & Medicinal Chemistry Letters, v.24, no.1, pp.209 - 213 Bioorganic & Medicinal Chemistry Letters 24 1 209 213 scie scopus 000329114200039 2-s2.0-84891491944 Chemistry, Medicinal Chemistry, Organic Pharmacology & Pharmacy Chemistry Article DNA-POLYMERASE TRIPHOSPHATES NUCLEOTIDES Sequencing-by-synthesis Reversible terminator Modified nucleotide DNA polymerase Triphosphate