Roh, Dae-Hyun Yoon, Seo-Yeon 2024-01-20T10:34:12Z 2024-01-20T10:34:12Z 2022-01-25 2014-01 0918-6158 https://pubs.kist.re.kr/handle/201004/127245 Sigma-1 receptors (Sig-1Rs) play a role in different types of pain and in central sensitization mechanism in spinal cord. However, it is currently unexplored whether Sig-1Rs are involved in orofacial pain processing. Here we show whether a selective Sig-1R antagonist, BD1047 reduces nociceptive responses in the mouse orofacial formalin model and the number of Fos-immunoreactive (ir) cells in the trigeminal nucleus caudalis (TNC). In addition, it was examined whether the phosphorylation of extracellular signal-regulated kinase (pERK) or p38 (pp38) mitogen-activated protein kinases (MAPK), which are closely linked to pain signaling and sensitization, in TNC was modified by BD1047. The 5% formalin (10 mu L) was subcutaneously injected into the right upper lip, and the rubbing responses with ipsilateral fore- or hind paw were counted for 45 min. BD1047 (1, 3 or 10 mg/kg) were intraperitoneally treated 30 min before formalin injection. High dose of BD1047 (10 mg/kg) produced significant anti-nociceptive effects in the first and the second phase. The number of Fos-ir cells in ipsilateral side of TNC was also reduced by BD1047 as compared to that in saline-treated animals. In addition, the number of pp38-ir cells in ipsilateral TNC was decreased in BD1047-treated animals, whereas the number of pERK-ir cells was not modified. Collectively, these results demonstrate that Sig-1Rs play a pivotal role in the orofacial pain processing, and the pp38 signaling pathway can be associated with Sig-1R's action in TNC. English PHARMACEUTICAL SOC JAPAN Sigma-1 Receptor Antagonist, BD1047 Reduces Nociceptive Responses and Phosphorylation of p38 MAPK in Mice Orofacial Formalin Model Article 10.1248/bpb.b13-00690 1 BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.37, no.1, pp.145 - 151 BIOLOGICAL & PHARMACEUTICAL BULLETIN 37 1 145 151 N scie scopus 000329000400021 2-s2.0-84892605618 Pharmacology & Pharmacy Pharmacology & Pharmacy Article ACTIVATED PROTEIN-KINASE SPINAL MICROGLIA CONTRIBUTES NEUROPATHIC PAIN MECHANICAL ALLODYNIA TRIGEMINAL NOCICEPTION NEUROACTIVE STEROIDS FOS EXPRESSION NITRIC-OXIDE RATS INDUCTION sigma-1 receptor orofacial pain formalin test Fos protein p38