Son, Hyun-Hwa Moon, Ju-Yeon Seo, Hong Seog Kim, Hyun Hee Chung, Bong Chul Choi, Man Ho 2024-01-20T10:34:31Z 2024-01-20T10:34:31Z 2021-09-05 2014-01 0022-2275 https://pubs.kist.re.kr/handle/201004/127261 Alterations of cholesterol metabolism are responsible for vasospastic angina and atherosclerosis. To comprehensively evaluate cholesterol metabolism, 18 sterols, including cholesterol, 6 cholesteryl esters (CEs), 3 cholesterol precursors, and 8 hydroxycholesterols (OHCs), were simultaneously analyzed using hybrid solid-phase extraction (SPE) purification coupled to high-temperature gas chromatography-mass spectrometry (HTGC-MS). Methanol-based hybrid SPE increased the selective extraction, and HTGC resulted in a good chromatographic resolution for the separation of lipophilic compounds. The limits of quantification of cholesterol and CEs ranged from 0.2 to 10.0 mu g/ml, while OHCs and cholesterol precursors ranged from 0.01 to 0.10 mu g/ml. Linearity as the correlation coefficient was higher than 0.99 with the exception of cholesteryl laurate, myristate, oleate, and linoleate (r(2) > 0.98). The precision (%coefficient of variation) and accuracy (%bias) ranged from 1.1 to 9.8% and from 75.9 to 125.1%, respectively. The overall recoveries of CEs ranged from 26.1 to 64.0%, and the recoveries of other sterols ranged from 83.8 to 129.3%. The cholesterol signatures showed sex differences in patients with vasospastic angina and may associate with 24-reductases. This technique can be useful for making clinical diagnoses and for an increased understanding of the pathophysiology of vasospastic angina. English AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC MASS-SPECTROMETRY METHOD LIQUID-CHROMATOGRAPHY FOLLOW-UP CARDIOVASCULAR-DISEASE HUMAN PLASMA OXYSTEROLS ESTER QUANTIFICATION PHOSPHOLIPIDS STEROLS High-temperature GC-MS-based serum cholesterol signatures may reveal sex differences in vasospastic angina Article 10.1194/jlr.D040790 1 JOURNAL OF LIPID RESEARCH, v.55, no.1, pp.155 - 162 JOURNAL OF LIPID RESEARCH 55 1 155 162 scie scopus 000330535100016 2-s2.0-84891350519 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article MASS-SPECTROMETRY METHOD LIQUID-CHROMATOGRAPHY FOLLOW-UP CARDIOVASCULAR-DISEASE HUMAN PLASMA OXYSTEROLS ESTER QUANTIFICATION PHOSPHOLIPIDS STEROLS hybrid solid-phase extraction-precipitation cardiovascular disease hydroxycholesterol