Shin, Yong-Kyu Ye, Michael B. Kim, Sung-Won Lee, Soo-Chan Kim, Il-Woung Kim, Su-Nam Yang, Hyun-Ok Kim, Si-Kwan 2024-01-20T10:34:53Z 2024-01-20T10:34:53Z 2021-09-05 2014-01 1756-4646 https://pubs.kist.re.kr/handle/201004/127280 This study intended to develop anti-obesity compounds from natural sources. In our screening program for anti-obesity agents, a compound isolated from Cordyceps militaris was found to be a potent inhibitor of the differentiation of 3T3-L1 pre-adipocytes. The active ingredient of C. militaris was isolated from methanol extracts by chromatography, re-crystallized in water, and subsequently identified as 3'-deoxyadenosine, otherwise known as cordycepin. Cordycepin was a potent inhibitor of cellular differentiation and triacylglycerol (TAG) synthesis at a concentration of 32 mu M without any signs of cytotoxicity. However, the inhibitory action was attenuated by supplementation with adenosine. Western blot analysis revealed that cordycepin down-regulated the protein levels of C/EBP alpha, PPAR-gamma, and anti-leptin; these proteins are known to be associated with TAG synthesis. Cordycepin-induced decrease in protein expression was countered by the addition of adenosine. In vitro pharmacokinetic studies demonstrate that 95% of the cordycepin (64 mu M initial concentration) added to the culture medium of 3T3-L1 cells was cleared within 6 h. The metabolic rate of cordycepin in vitro was similar to that of adenosine, suggesting that cordycepin inhibits the differentiation of pre-adipocytes and blocks the synthesis of TAG in 3T3-L1 cells by interfering with adenosine metabolism. (C) 2014 Published by Elsevier Ltd. English ELSEVIER SCIENCE BV CORDYCEPS-SINENSIS SCIENTIFIC REDISCOVERY PPAR-GAMMA MILITARIS OBESITY PREVALENCE MEDICINE RECEPTOR WEIGHT 3 '-Deoxyadenosine inhibits pre-adipocyte differentiation and biosynthesis of triacylglycerol in 3T3-L1 cells Article 10.1016/j.jff.2013.10.024 1 JOURNAL OF FUNCTIONAL FOODS, v.6, pp.331 - 338 JOURNAL OF FUNCTIONAL FOODS 6 331 338 scie scopus 000331423000033 2-s2.0-84895072168 Food Science & Technology Nutrition & Dietetics Food Science & Technology Nutrition & Dietetics Article CORDYCEPS-SINENSIS SCIENTIFIC REDISCOVERY PPAR-GAMMA MILITARIS OBESITY PREVALENCE MEDICINE RECEPTOR WEIGHT 3T3-L1 cells Cordycepin Differentiation Triacylglycerol Anti-obesity In vitro pharmacokinetics