Song, Mi-Kyung Choi, Han-Seam Lee, Hyo-Sun Kim, Youn-Jung Park, Yong-Keun Ryu, Jae-Chun 2024-01-20T11:00:34Z 2024-01-20T11:00:34Z 2021-09-04 2014-01 1382-6689 https://pubs.kist.re.kr/handle/201004/127307 Previous environmental microRNA (miRNA) studies have investigated a limited number of candidate miRNAs and have not evaluated functional effects on gene expression. In this study, we aimed to identify octanal (OC)-sensitive miRNAs and to characterize the relationships between miRNAs and expression of candidate genes involved in OC-induced toxicity. Microarray analysis identified 15 miRNAs that were differentially expressed in OC-exposed A549 human alveolar cells. Integrated analyses of miRNA and mRNA expression profiles identified significant miRNA-mRNA anti-correlations. GO analysis of 101 putative target genes showed that the biological category 'MAPK signaling pathway' was prominently annotated. Moreover, we detected increased phosphorylation of p38 MAPK in the OC-exposed group. By integrating the transcriptome and microRNAome, we provide evidence that OC can affect MAPK-induced toxicity signaling. Therefore, this study demonstrates the added value of an integrated miRNA-mRNA approach for identifying molecular events induced by environmental pollutants in an in vitro human model (C) 2013 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV GENE-EXPRESSION MAMMALIAN MICRORNAS RAT-LIVER FORMALDEHYDE KINASE INDUCTION CANCER P38 INFLAMMATION ACTIVATION Analysis of microRNA and mRNA expression profiles highlights alterations in modulation of the MAPK pathway under octanal exposure Article 10.1016/j.etap.2013.11.005 1 ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, v.37, no.1, pp.84 - 94 ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 37 1 84 94 scie scopus 000332919800010 2-s2.0-84889584900 Environmental Sciences Pharmacology & Pharmacy Toxicology Environmental Sciences & Ecology Pharmacology & Pharmacy Toxicology Article GENE-EXPRESSION MAMMALIAN MICRORNAS RAT-LIVER FORMALDEHYDE KINASE INDUCTION CANCER P38 INFLAMMATION ACTIVATION MicroRNA Target mRNAs Aldehydes Octanal Mitogen-activated protein kinase Microarray