Koo, Heebeom Min, Kyung Hyun Lee, Sang Cheon Park, Jae Hyung Park, Kinam Jeong, Seo Young Choi, Kuiwon Kwon, Ick Chan Kim, Kwangmeyung 2024-01-20T11:00:44Z 2024-01-20T11:00:44Z 2021-09-05 2013-12-28 0168-3659 https://pubs.kist.re.kr/handle/201004/127315 Enhanced drug-loading and therapeutic efficacies are highly essential properties for nanoparticles as tumor-targeting drug carriers. Herein, we developed the glycol chitosan nanoparticles with hydrotropic oligomers (HO-CNPs) as a new tumor targeting drug delivery system. For enhancing drug-loading efficiency of paclitaxel in drug carriers, hydrotropic 2-(4-(vinylbenzyloxy)-N,N-diethylnicotinamide) (VBODENA-COOH) oligomers, that were used for enhancing the aqueous solubility of paclitaxel, were directly conjugated to glycol chitosan polymers. The amphiphilic conjugates readily formed nanoparticle structure (average size = 302 +/- 22 nm) in aqueous condition. Water-insoluble paclitaxel (PTX) was readily encapsulated into HO-CNPs with a high drug-loading amount up to 24.2 wt.% (2.4 fold higher than other polymeric nanoparticles) by a simple dialysis method. The PTX encapsulated HO-CNPs (PTX-HO-CNPs; average size = 343 +/- 12 nm) were very stable in aqueous media up to 50 days. Also, PTX-HO-CNPs presented rapid cellular uptake and lower cytotoxicity in cell culture system, compared to Cremophor EL/ethanol formulation of PTX. In tumor-bearing mice, the extravasation and accumulation of PTX-HO-CNPs in tumor tissue were precisely observed by intravital fluorescence imaging techniques. Furthermore, PTX-HO-CNPs showed the higher therapeutic efficacy, compared to Abraxane (R), a commercialized PTX-formulation. These overall results demonstrate its potential as a new nano-sized PTX carrier for cancer treatment. (C) 2013 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV HYALURONIC-ACID NANOPARTICLES IN-VIVO STABILITY DESIGN Enhanced drug-loading and therapeutic efficacy of hydrotropic oligomer-conjugated glycol chitosan nanoparticles for tumor-targeted paclitaxel delivery Article 10.1016/j.jconrel.2013.08.297 1 JOURNAL OF CONTROLLED RELEASE, v.172, no.3, pp.823 - 831 JOURNAL OF CONTROLLED RELEASE 172 3 823 831 scie scopus 000328492800025 2-s2.0-84887132067 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article HYALURONIC-ACID NANOPARTICLES IN-VIVO STABILITY DESIGN Hydrotropic oligomer Glycol chitosan nanoparticles Tumor-targeting Paclitaxel Drug delivery Cancer therapy