Lee, Soojin Kim, Borim Jeong, Daun Oh, Minkyu Um, Youngsoon Kim, Young-Rok Kim, Jungwook Lee, Jinwon 2024-01-20T11:01:54Z 2024-01-20T11:01:54Z 2021-09-05 2013-12 0168-1656 https://pubs.kist.re.kr/handle/201004/127373 Microorganisms that produce 2,3-butanediol (2,3-BDO) are mostly mixed acid fermentation microorganisms, and they synthesize 2,3-BDO in order to suppress medium acidification. The 2,3-BDO operon (budBAC) is activated by the LysR regulator protein derived from the budR. In this study, the effect of the budR on 2,3-BDO-biosynthesis was observed at gene transcription level. The Klebsiella pneumoniae strains (wabG-deleted strain (SGSB100), budR over-expressed strain (SGSB101), and the budR-deleted strain (SGSB102)) were constructed. The resulting strains were cultivated in unified conditions. Samples were obtained at the lag-, log-, and stationary-phase of cell growth, and gene transcription levels of the budR, 2,3-BDO-biosynthesis-related (budB, budA, and budC), and acid-biosynthesis-related (IdhA and ack) genes were observed. During the lag-phase of cell growth in SGSB101, the budR transcription level increased approximately 8-fold, and 2,3-BDO production increased approximately 2-fold, when compared to SGSB100. Also in SGSB101 the transcription level of the acid-biosynthesis-related genes (IdhA and ack) increased approximately up to 11-fold during the lag-phase of cell growth compared to SGSB100. On contrast, in SGSB102 budR transcription was not detected, and the transcription level of the acid-biosynthesis-related genes (IdhA and ack) decreased approximately 70-fold during the lag-phase of cell growth compared to SGSB100. This is by far the first observation of 2,3-BDO regulation mechanism at gene transcription level in K. pneumoniae, and therefore may be useful for understanding and improving 2,3-BDO biosynthesis metabolic network. (C) 2013 Elsevier B.V. All rights reserved. English Elsevier BV Observation of 2,3-butanediol biosynthesis in Lys regulator mutated Klebsiella pneurnoniae at gene transcription level Article 10.1016/j.jbiotec.2013.09.015 1 Journal of Biotechnology, v.168, no.4, pp.520 - 526 Journal of Biotechnology 168 4 520 526 N scie scopus 000327843200032 2-s2.0-84888832089 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article FERMENTATION PATHWAY MICROBIAL-PRODUCTION BACILLUS-SUBTILIS PNEUMONIAE DEHYDROGENASE METABOLISM BUTANEDIOL TERRIGENA BACTERIA OXYTOCA Putative LysR-family transcriptional regulator budR Klebsiella pneumoniae 2,3-Butanediol Real-time PCR