Ryu, Ju Hee Shin, Miyoung Kim, Sun Ah Lee, Sangmin Kim, Hyunjoon Koo, Heebeom Kim, Byung-Soo Song, Hyun Kyu Kim, Sun Hwa Choi, Kuiwon Kwon, Ick Chan Jeon, Hyesung Kim, Kwangmeyung 2024-01-20T11:02:08Z 2024-01-20T11:02:08Z 2021-09-05 2013-12 0142-9612 https://pubs.kist.re.kr/handle/201004/127385 Receptor-targeted imaging is emerging as a promising strategy for diagnosis of human cancer. Herein, we developed an epidermal growth factor-based nanoprobe (EGF-NP) for in vivo optical imaging of epidermal growth factor receptor (EGFR), an important target for cancer imaging. The self-quenched EGF-NP is fabricated by sequentially conjugating a near-infrared (NIR) fluorophore (Cy5.5) and a quencher (BHQ-3) to EGF, a low-molecular weight polypeptide (6.2 kDa), compared to EGFR antibody (150 kDa). The self-quenched EGF-NP presented great specificity to EGFR, and rapidly internalized into the cells, as monitored by time-lapse imaging. Importantly, the self-quenched EGF-NP boosted strong fluorescence signals upon EGFR-targeted uptake into EGFR-expressing cells, followed by lysosomal degradation, as confirmed by lysosomal marker cell imaging. Consistent with cellular results, intravenous injection of EGF-NP into tumor-bearing mice induced strong NIR fluorescence intensity in the target tumor tissue with high specificity against EGFR-expressing cancer cells. Signal accumulation of EGF-NP in tumor was much faster than that of EGFR monoclonal antibody (Cetuximab)-Cy5.5 conjugates due to the rapid clearance from the body and tissue permeability of low-molecular weight EGF. This self-quenched, EGF-based imaging probe can be applied for diagnosis of various cancers. (C) 2013 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD GOLD NANOPARTICLES BREAST-CANCER THERAPY EXPRESSION DELIVERY TUMORS CONJUGATE CARCINOMA LIGANDS MODELS In vivo fluorescence imaging for cancer diagnosis using receptor-targeted epidermal growth factor-based nanoprobe Article 10.1016/j.biomaterials.2013.08.026 1 BIOMATERIALS, v.34, no.36, pp.9149 - 9159 BIOMATERIALS 34 36 9149 9159 scie scopus 000326426500018 2-s2.0-84883753482 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article GOLD NANOPARTICLES BREAST-CANCER THERAPY EXPRESSION DELIVERY TUMORS CONJUGATE CARCINOMA LIGANDS MODELS Epidermal growth factor receptor (EGFR) EGF-based nanoprobe Cancer imaging Receptor-targeted imaging Epidermal growth factor (EGF)