Ha, Chang Man Hwang, Eun Mi Kim, Eunju Lee, Da Yong Chang, Sunghoe Lee, Byung Ju Hong, Seong-Geun Park, Jae-Yong 2024-01-20T11:02:31Z 2024-01-20T11:02:31Z 2021-09-04 2013-12 1016-8478 https://pubs.kist.re.kr/handle/201004/127406 Neural epidermal growth factor-like protein-like 2 (NELL2) is a secreted glycoprotein that is predominantly expressed in the nervous system, but little is known about the intracellular movement and secretion mechanism of this protein. By monitoring the localization and movements of enhanced green fluorescent protein (EGFP)-labeled NELL2 in living cultured hippocampal neuroprogenitor HiB5 cells, we determined the subcellular localization of NELL2 and its intracellular movement and secretion mechanism. Cterminal EGFP-fused NELL2 showed a typical expression pattern of secreted proteins, especially with respect to its localization in the endoplasmic reticulum, Golgi apparatus, and punctate structures. Vesicles containing NELL2 exhibited bidirectional movement in HiB5 cells. The majority of the vesicles (70.1%) moved in an anterograde direction with an average velocity of 0.454 mu m/s, whereas some vesicles (28.7%) showed retrograde movement with an average velocity of 0.302 mu m/s. The movement patterns of NELL2 vesicles were dependent upon the presence of microtubules in HiB5 cells. Anterograde movement of NELL2 did not lead to a detectable accumulation of NELL2 in the peripheral region of the cell, indicating that it was secreted into the culture medium. We also showed that the N-terminal 29 amino acids of NELL2 were important for secretion of this protein. Taken together, these results strongly suggest that the N-terminal region of NELL2 determines both the pattern of its intracellular expression and transport of NELL2 vesicles by high-velocity movement. Therefore, NELL2 may affect the cellular activity of cells in a paracrine or autocrine manner. English KOREAN SOC MOLECULAR & CELLULAR BIOLOGY EGF-LIKE REPEATS FAMILY PROTEIN RAT-BRAIN IN-VIVO MOTOR NEURONS TRANSPORT DIFFERENTIATION KIF3A/B DOMAIN The molecular mechanism of NELL2 movement and secretion in hippocampal progenitor HiB5 cells Article 10.1007/s10059-013-0216-5 1 MOLECULES AND CELLS, v.36, no.6, pp.527 - 533 MOLECULES AND CELLS 36 6 527 533 scie scopus kci 000328856300005 2-s2.0-84900311744 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article EGF-LIKE REPEATS FAMILY PROTEIN RAT-BRAIN IN-VIVO MOTOR NEURONS TRANSPORT DIFFERENTIATION KIF3A/B DOMAIN glycoprotein intracellular movement NELL2 secretion