Park, Junghong Kim, Jihoon Singha, Kaushik Han, Dong-Keun Park, Hansoo Kim, Won Jong 2024-01-20T11:04:28Z 2024-01-20T11:04:28Z 2021-09-04 2013-11 0142-9612 https://pubs.kist.re.kr/handle/201004/127505 The work demonstrated a successful synthesis of nitric oxide (NO)-releasing material and its antibacterial effect on Gram-negative Escherichia coli (E. coli), Gram-positive Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA). The polymeric support composed of thermosensitive Pluronic F68 having good biocompatibility and branched polyethylenimine (BPEI) housed N-diazeniumdiolates (NONOates) which could store and release NO under appropriate physiological condition. The developed F68-BPEI-NONOates releases a sufficient amount of NO under physiological condition to elicit effective killing of E. coli, S. aureus and MRSA. The antibacterial ability of the released NO was compared to un-treated control or unmodified F68 polymer by using confocal microscopy; F68-BPEI-NONOates demonstrated excellent antibacterial activity with in vitro low cytotoxicity. TEM investigation also revealed the destruction of bacteria membrane caused by NO. The effectiveness of F68-BPEI-NONOates against resistant strains such as MRSA provides a very simple but highly efficient strategy to combat drug-resistant bacterial infections. (C) 2013 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD RESISTANT STAPHYLOCOCCUS-AUREUS ESCHERICHIA-COLI MAGNETIC NANOPARTICLES SILVER ION DELIVERY PEROXYNITRITE BACTERIA ENCAPSULATION MECHANISMS DENDRIMERS Nitric oxide integrated polyethylenimine-based tri-block copolymer for efficient antibacterial activity Article 10.1016/j.biomaterials.2013.07.064 1 BIOMATERIALS, v.34, no.34, pp.8766 - 8775 BIOMATERIALS 34 34 8766 8775 scie scopus 000324450600021 2-s2.0-84882814101 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article RESISTANT STAPHYLOCOCCUS-AUREUS ESCHERICHIA-COLI MAGNETIC NANOPARTICLES SILVER ION DELIVERY PEROXYNITRITE BACTERIA ENCAPSULATION MECHANISMS DENDRIMERS N-Diazeniumdiolates Nitric oxide delivery Antibacterial activity Methicillin-resistance