Jho, Eun Hye Kang, Kyungsu Oidovsambuu, Sarangerel Lee, Eun Ha Jung, Sang Hoon Shin, Il-Shik Nho, Chu Won 2024-01-20T11:04:56Z 2024-01-20T11:04:56Z 2021-09-05 2013-10-31 1976-6696 https://pubs.kist.re.kr/handle/201004/127528 We investigated the protective effects of Gymnaster koraiensis against oxidative stress-induced hepatic cell damage. We used two different cytotoxicity models, i.e., the administration of tert-butyl hydroperoxide (t-BHP) and acetaminophen, in HepG2 cells to evaluate the protective effects of G. koraiensis. The ethyl acetate (EA) fraction of G. koraiensis and its major compound, 3,5-di-O-caffeoylquinic acid (DCQA), exerted protective effects in the t-BHP-induced liver cytotoxicity model. The EA fraction and DCQA ameliorated t-BHP-induced reductions in GSH levels and exhibited free radical scavenging activity. The EA fraction and DCQA also significantly reduced t-BHP-induced DNA damage in HepG2 cells. Furthermore, the hexane fraction of G. koraiensis and its major compound, gymnasterkoreayne B (GKB), exerted strong hepatoprotection in the acetaminophen-induced cytotoxicity model. CYP 3A4 enzyme activity was strongly inhibited by the extract, hexane fraction, and GKB. The hexane fraction and GKB ameliorated acetaminophen-induced reductions in GSH levels and protected against cell death. English KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY SINGLE-STRAND BREAKAGE BENZOQUINONE IMINE HUMAN HEPATOCYTES BUTYLHYDROPEROXIDE HEPATOTOXICITY MECHANISMS TOXICITY OXIDANTS DISEASES STRESS Gymnaster koraiensis and its major components, 3,5-di-O-caffeoylquinic acid and gymnasterkoreayne B, reduce oxidative damage induced by tert-butyl hydroperoxide or acetaminophen in HepG2 cells Article 10.5483/BMBRep.2013.46.10.037 1 BMB REPORTS, v.46, no.10, pp.513 - 518 BMB REPORTS 46 10 513 518 scie scopus kci ART001813076 000326327300007 2-s2.0-84886783391 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article SINGLE-STRAND BREAKAGE BENZOQUINONE IMINE HUMAN HEPATOCYTES BUTYLHYDROPEROXIDE HEPATOTOXICITY MECHANISMS TOXICITY OXIDANTS DISEASES STRESS Gymnaster koraiensis Gymnasterkoreayne B Hepatoprotection HepG2 Wild vegetable