Ku, Kang Mo Choi, Jeong Hee Kim, Hyoung Seok Kushad, Mosbah M. Jeffery, Elizabeth H. Juvik, John A. 2024-01-20T11:30:16Z 2024-01-20T11:30:16Z 2021-08-31 2013-10-16 1932-6203 https://pubs.kist.re.kr/handle/201004/127543 Effect of pre-harvest methyl jasmonate (MeJA) and post-harvest 1-methylcyclopropene (1-MCP) treatments on broccoli floret glucosinolate (GS) concentrations and quinone reductase (QR, an in vitro anti-cancer biomarker) inducing activity were evaluated two days prior to harvest, at harvest and at 10, 20, and 30 days of post-harvest storage at 4 C. MeJA treatments four days prior to harvest of broccoli heads was observed to significantly increase floret ethylene biosynthesis resulting in chlorophyll catabolism during post-harvest storage and reduced product quality. Post-harvest treatment with 1-methylcyclopropene (1-MCP), which competitively binds to protein ethylene receptors, maintained post-harvest floret chlorophyll concentrations and product visual quality in both control and MeJA-treated broccoli. Transcript abundance of BoPPH, a gene which is responsible for the synthesis of pheophytinase, the primary enzyme associated with chlorophyll catabolism in broccoli, was reduced by 1-MCP treatment and showed a significant, negative correlation with floret chlorophyll concentrations. The GS, glucobrassicin, neoglucobrassicin, and gluconasturtiin were significantly increased by MeJA treatments. The products of some of the GS from endogenous myrosinase hydrolysis [sulforaphane (SF), neoascorbigen (NeoASG), N-methoxyindole-3-carbinol (NI3C), and phenethyl isothiocyanate (PEITC)] were also quantified and found to be significantly correlated with QR. Sulforaphane, the isothiocyanate hydrolysis product of the GS glucoraphanin, was found to be the most potent QR induction agent. Increased sulforaphane formation from the hydrolysis of glucoraphanin was associated with up-regulated gene expression of myrosinase (BoMyo) and the myrosinase enzyme co-factor gene, epithiospecifier modifier1 (BoESM1). This study demonstrates the combined treatment of MeJA and 1-MCP increased QR activity without post-harvest quality loss. English PUBLIC LIBRARY SCIENCE CHLOROPHYLL DEGRADING GENES CELL-CYCLE ARREST COLON-CANCER GLUCOSINOLATE PROFILES MYROSINASE ACTIVITY MOLECULAR-CLONING ETHYLENE-RESPONSE ARABIDOPSIS EXPRESSION ISOTHIOCYANATES Methyl Jasmonate and 1-Methylcyclopropene Treatment Effects on Quinone Reductase Inducing Activity and Post-Harvest Quality of Broccoli Article 10.1371/journal.pone.0077127 1 PLOS ONE, v.8, no.10 PLOS ONE 8 10 scie scopus 000326019400095 Multidisciplinary Sciences Science & Technology - Other Topics Article CHLOROPHYLL DEGRADING GENES CELL-CYCLE ARREST COLON-CANCER GLUCOSINOLATE PROFILES MYROSINASE ACTIVITY MOLECULAR-CLONING ETHYLENE-RESPONSE ARABIDOPSIS EXPRESSION ISOTHIOCYANATES vegetable human health promotion glucosinolate