Huh, Yeowool Cho, Jeiwon 2024-01-20T11:30:21Z 2024-01-20T11:30:21Z 2021-09-04 2013-10-10 1662-5153 https://pubs.kist.re.kr/handle/201004/127547 Anesthetics are often used to characterize the activity of single neurons in vivo for their advantages such as reduction of noise level and convenience in noxious stimulations. Urethane has been a widely used anesthetic in thalamic studies under the assumption that sensory signals are still relayed to the thalamus under urethane anesthesia and that thalamic response would therefore reflect the response of the awake state. We tested this assumption by comparing thalamic activity in terms of tonic and burst firing modes during "the awake state" or under "urethane anesthesia" using the extracellular single unit recording technique. We first tested how thalamic relay neurons respond to the introduction of urethane, and then tested how urethane influences thalamic discharges under formalin-induced nociception. Urethane significantly depressed overall firing rates of thalamic relay neurons, which was sustained despite the delayed increase of burst activity over a 4 h recording period. Thalamic response to nociception under anesthesia was also similar overall except for the slight and transient increase of burst activity. Overall, results demonstrated that urethane suppresses the activity of thalamic relay neurons and that, despite the slight fluctuation of burst firing, formalin-induced nociception cannot significantly change the firing pattern of thalamic relay neurons that was caused by urethane. English FRONTIERS MEDIA SA FORMALIN TEST OSCILLATIONS RATS Urethane anesthesia depresses activities of thalamocortical neurons and alters its response to nociception in terms of dual firing modes Article 10.3389/fnbeh.2013.00141 1 FRONTIERS IN BEHAVIORAL NEUROSCIENCE, v.7 FRONTIERS IN BEHAVIORAL NEUROSCIENCE 7 scie scopus 000325460800001 2-s2.0-84886256193 Behavioral Sciences Neurosciences Behavioral Sciences Neurosciences & Neurology Article FORMALIN TEST OSCILLATIONS RATS urethane thalamus nociception anesthesia bursting activity tonic activity single unit recording