Vinegoni, Claudio Lee, Sungon Fumene Feruglio, Paolo Marzola, Pasquina Nahrendorf, Matthias Weissleder, Ralph 2024-01-20T11:30:30Z 2024-01-20T11:30:30Z 2021-09-05 2013-10-01 2156-7085 https://pubs.kist.re.kr/handle/201004/127554 In vivo imaging is often severely compromised by cardiovascular and respiratory motion. Highly successful motion compensation techniques have been developed for clinical imaging (e.g. magnetic resonance imaging) but the use of more advanced techniques for intravital microscopy is largely unexplored. Here, we implement a sequential cardiorespiratory gating scheme (SCG) for averaged microscopy. We show that SCG is very efficient in eliminating motion artifacts, is highly practical, enables high signal-to-noise ratio (SNR) in vivo imaging, and yields large field of views. The technique is particularly useful for high-speed data acquisition or for imaging scenarios where the fluorescence signal is not significantly above noise or background levels. (c) 2013 Optical Society of America English OPTICAL SOC AMER Sequential average segmented microscopy for high signal-to-noise ratio motion-artifact-free in vivo heart imaging Article 10.1364/BOE.4.002095 1 BIOMEDICAL OPTICS EXPRESS, v.4, no.10, pp.2095 - 2106 BIOMEDICAL OPTICS EXPRESS 4 10 2095 2106 scie scopus 000325140800025 2-s2.0-84884944078 Biochemical Research Methods Optics Radiology, Nuclear Medicine & Medical Imaging Biochemistry & Molecular Biology Optics Radiology, Nuclear Medicine & Medical Imaging Article