Lee, Sang-Hoon Park, Jeong-Hoon Kang, Hyun-Jin Lee, Young Haeng Lee, Taek Jun Park, Hee-Deung 2024-01-20T11:31:20Z 2024-01-20T11:31:20Z 2021-09-05 2013-10 0960-8524 https://pubs.kist.re.kr/handle/201004/127598 To investigate the distribution and abundance of spirochaetal communities within anaerobic digesters, pyrosequencing of the 16S rRNA gene was conducted. Phylogenetic analysis identified a cluster which included the majority of core spirochaetal operational taxonomic units (OTUs) and environmental clones but no pure-culture strains. Distribution of the core OTUs demonstrated an importance of local factors in shaping the structure of Spirochaetes. Spirochaetal to bacterial 16S rRNA gene copy numbers varied from 1.3% to 30.0% depending on digester samples. Environmental variables were found to influence the relative abundance of Spirochaetes. In a batch anaerobic digestion experiment testing the response to different substrates, acetate most stimulated the activity of Spirochaetes, suggesting possible acetate oxidation by syntrophic acetate oxidation process. Taken together, the results obtained in this study provides an insight into the ecology of Spirochaetes in anaerobic digesters and a basis for future studies examining ecological function of these bacteria. (c) 2013 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD MICROBIAL DIVERSITY COMMUNITY STRUCTURE SLUDGE DIGESTER BACTERIAL SEQUENCES ARCHAEAL ACETATE Distribution and abundance of Spirochaetes in full-scale anaerobic digesters Article 10.1016/j.biortech.2013.02.070 1 BIORESOURCE TECHNOLOGY, v.145, pp.25 - 32 BIORESOURCE TECHNOLOGY 145 25 32 scie scopus 000324899600005 2-s2.0-84883597098 Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels Agriculture Biotechnology & Applied Microbiology Energy & Fuels Article; Proceedings Paper MICROBIAL DIVERSITY COMMUNITY STRUCTURE SLUDGE DIGESTER BACTERIAL SEQUENCES ARCHAEAL ACETATE Anaerobic digestion Spirochaetes Microbial community Pyrosequencing Syntrophic acetate oxidation