Kim, Seil Gong, Gyeongtaek Park, Tai Hyun Um, Youngsoon 2024-01-20T11:31:51Z 2024-01-20T11:31:51Z 2021-09-04 2013-10 1466-5026 https://pubs.kist.re.kr/handle/201004/127626 An obligately aerobic, chemoheterotrophic, mesophilic prosthecate bacterium, designated strain CGM1-3EN(T), was isolated from the enrichment cultures of forest soil from Cheonggyesan Mountain, Republic of Korea. Cells were Gram-reaction-negative, motile rods (1.3-2.4 mu m long by 0.30-0.75 mu m wide) with single flagella. The strain grew at 10-37 degrees C (optimum 25-30 degrees C) and at pH 4.5-9.5 (optimum 5.0-7.0). The major cellular fatty acids were C-16:0, C-18:1 omega 7c 11-methyl, C-12:1 3-OH and summed feature 8 (comprising C-18:1 omega 7c/C-18:1 omega 6c). The genomic DNA G + C content of strain CGM1-3EN(T) was 63.7 mol%. The closest phylogenetic neighbour to strain CGM1-3EN(T) was identified as Asticcacaulis biprosthecium DSM 4723(T) (97.2 % 16S rRNA gene sequence similarity) and the DNA-DNA hybridization value between strain CGM1-3EN(T) and A. biprosthecium DSM 4723(T) was less than 24.5 %. Strain CGM1-3EN(T) used D-glucose, D-fructose, sucrose, maltose, trehalose, D-mannose, D-mannitol, D-sorbitol, D-galactose, cellobiose, lactose, raffinose, fumarate, pyruvate, DL-alanine and glycerol as carbon sources. Based on data from the present polyphasic study, the forest soil isolate CGM1-3EN(T) is considered to represent a novel species of the genus Asticcacaulis, for which the name Asticcacaulis solisilvae sp. nov. is proposed. The type strain is CGM1-3EN(T) (=AIM0088(T)=KCTC 32102(T)=JCM 18544(T)). English Society for General Microbiology Asticcacaulis solisilvae sp nov., isolated from forest soil Article 10.1099/ijs.0.047423-0 1 International Journal of Systematic and Evolutionary Microbiology, v.63, pp.3829 - 3834 International Journal of Systematic and Evolutionary Microbiology 63 3829 3834 N scie scopus 000327910200047 2-s2.0-84885193433 Microbiology Microbiology Article RNA GENE-SEQUENCES RENATURATION RATES DNA HYBRIDIZATION CAULOBACTER PHENYLOBACTERIUM BACTERIUM EZTAXON Asticcacaulis soil isolation