Haque, M.M. Im, H.-Y. Seo, J.-E. Hasan, M. Woo, K. Kwon, O.-S. 2024-01-20T11:32:00Z 2024-01-20T11:32:00Z 2021-09-02 2013-10 2093-5552 https://pubs.kist.re.kr/handle/201004/127634 Nano-sized materials are increasingly used in cosmetics, diagnosis, imaging, and drug delivery. It also involved in specific functionality with lymphoid systems. However, the toxicity and mechanisms of quantum dots (QDs) uptake into mammalian cells are poorly understood. Our study was to investigate the toxicity and tissue uptake of polyethylene glycol-folic acid-conjugated (PEG-FA), and only polyethylene glycol-conjugated (PEG) cadmium selenide/cadmium sulfide (CdSe/CdS) QDs using precision-cut spleen slices of Sprague-Dawley (SD) rats. QDs were treated with different doses (0-300 nM) to the spleen of SD rats, and their toxic effects and tissue uptake were examined by LDH, NADPH oxidase, and histological analyses. No dose-dependent changes in LDH were observed. But high uptake of the QD-PEG-FA into spleen slices was observed by fluorescence microscopic examination in dose-dependent manner, while most of the QD-PEG was found on the edge of the slices. The NADPH oxidase activity was increased at high dose (300 nM) in both QD-PEG-FA- or QD-PEG-treated spleen slices indicating oxidative stresses. No damages were noticed in histological study confirming no toxicity in both types of QDs. Based on the above observations, we may conclude that surface coating property is an important factor in determining QDs uptake into mammalian cells. These findings provide insight into the specific mechanism of QDs uptake in cells. ？ 2013 The Korean Society of Pharmaceutical Sciences and Technology. English Effects of folic acid and polyethylene glycol coated quantum dots on toxicity and tissue uptake to precision-cut spleen slices of rats Article 10.1007/s40005-013-0082-3 1 Journal of Pharmaceutical Investigation, v.43, no.5, pp.375 - 383 Journal of Pharmaceutical Investigation 43 5 375 383 N scopus kci ART001812454 2-s2.0-84884571685 Article cadmium selenide cadmium sulfide decylamine folic acid lactate dehydrogenase macrogol octadecylamine quantum dot reduced nicotinamide adenine dinucleotide phosphate oxidase animal tissue article cell viability coated particle controlled study enzyme activity fluorescence histopathology nanotechnology nonhuman priority journal protein determination rat spleen CdSe/CdS quantum dots In vitro Precision-cut spleen slice Tissue uptake Toxicity