Kim, D. Lee, J.Y. Song, D.-G. Kwon, S. Lee, Y. Pan, C.-H. Kwon, H.-J. 2024-01-20T11:32:02Z 2024-01-20T11:32:02Z 2021-09-02 2013-10 2167-9436 https://pubs.kist.re.kr/handle/201004/127635 Post-translational modification regulated by conjugation of a small ubiquitin-like modifier (SUMO) is involved in various cellular processes. In this study, we expressed and purified recombinant human SUMO-1 (hSUMO-1). BALB/c mice were immunized with a complex of hSUMO-1 protein and Lipoplex(O) to produce hSUMO-1-specific antibodies. Using conventional hybridoma technology, we obtained four hybridoma clones derived from the mouse with the highest antibody titer against hSUMO-1. Based on Western blot analysis, our hSUMO-1 monoclonal antibody specifically recognizes hSUMO-1, but not other SUMO proteins. These results support that the anti-hSUMO-1 monoclonal antibody produced with the aid of Lipoplex(O) adjuvant is specific and that Lipoplex(O) is useful for development of monoclonal antibodies against recombinant protein. In addition, we analyzed human tissues to examine the distribution of hSUMO-1. Higher expression of hSUMO-1 was detected in normal adrenal gland, esophagus, pancreas, liver, stomach, kidney, and uterus than in corresponding cancer tissues, suggesting a tumor suppressive function of hSUMO-1. ？ 2013 Mary Ann Liebert, Inc. English CpG oligodeoxynucleotide DNA lipoplex liposome monoclonal antibody monoclonal antibody 1B12 E2 recombinant protein SUMO 1 protein unclassified drug animal cell animal experiment antibody titer article cancer tissue controlled study female human human tissue hybridoma immunization immunohistochemistry mouse nonhuman priority journal protein expression tissue microarray Western blotting Animals Antibodies, Monoclonal Blotting, Western DNA Primers Enzyme-Linked Immunosorbent Assay Gene Expression Profiling Humans Hybridomas Immunization Immunohistochemistry Mice Mice, Inbred BALB C Microarray Analysis Recombinant Proteins SUMO-1 Protein A monoclonal antibody against the human SUMO-1 protein obtained by immunization with recombinant protein and CpG-DNA-liposome complex Article 10.1089/mab.2013.0040 1 Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, v.32, no.5, pp.354 - 361 Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 32 5 354 361 scopus 2-s2.0-84885640850 Article CpG oligodeoxynucleotide DNA lipoplex liposome monoclonal antibody monoclonal antibody 1B12 E2 recombinant protein SUMO 1 protein unclassified drug animal cell animal experiment antibody titer article cancer tissue controlled study female human human tissue hybridoma immunization immunohistochemistry mouse nonhuman priority journal protein expression tissue microarray Western blotting Animals Antibodies, Monoclonal Blotting, Western DNA Primers Enzyme-Linked Immunosorbent Assay Gene Expression Profiling Humans Hybridomas Immunization Immunohistochemistry Mice Mice, Inbred BALB C Microarray Analysis Recombinant Proteins SUMO-1 Protein Monoclonal antibody SUMO-1 CpG-DNA-liposome complex