Hyun, Hyesun Lee, Kyuri Min, Kyung Hyun Jeon, Pureum Kim, Kwangmeyung Jeong, Seo Young Kwon, Ick Chan Park, Tae Gwan Lee, Minhyung 2024-01-20T11:32:26Z 2024-01-20T11:32:26Z 2021-09-04 2013-09-28 0168-3659 https://pubs.kist.re.kr/handle/201004/127653 Fluorescein-labeled hyaluronic acids (HA) were immobilized on gold nanoparticles for reactive oxygen species (ROS) detection. The efficacy of HA immobilized gold nanoparticles (HHAuNPs) was evaluated in a stroke animal model. The stroke rat model was produced by transient middle cerebral artery occlusion (MCAO), which induced transient ischemia and reperfusion (I/R) in the brain. The increase of ROS in the I/R brain was confirmed by TBARS assay with the brain extracts. For brain imaging, HHAuNPs were injected into the rat brain 1 h before transient MCAO. Five hours after the injection, the rats were sacrificed and the brains were subjected to imaging analysis. The results showed that stronger signals were detected in the I/R brains than in the normal brains. To identify the time window for effective detection of ROS, HHAuNPs were injected into the post-ischemic rat brains at various time points. The results showed that ROS level reached a maximum at 24 h after the transient MCAO. Also, a live imaging study was performed with HHAuNPs in the normal and I/R animals. The results confirmed that ROS level increased in the I/R animal group with time, while the signal was decreased in the normal animal group. Together, our results suggest that HHAuNPs may be useful to monitor ROS level in the ischemic brain and to identify the infarct areas in ischemic brains for the treatment of stroke. (C) 2013 Elsevier B. V. All rights reserved. English ELSEVIER SCIENCE BV MAGNETIC NANOPARTICLES OXIDATIVE STRESS DRUG DISCOVERY DIAGNOSTIC APPLICATIONS STROKE CANCER THERAPY DELIVERY GENE MICE Ischemic brain imaging using fluorescent gold nanoprobes sensitive to reactive oxygen species Article 10.1016/j.jconrel.2013.06.002 1 JOURNAL OF CONTROLLED RELEASE, v.170, no.3, pp.352 - 357 JOURNAL OF CONTROLLED RELEASE 170 3 352 357 scie scopus 000324040800006 2-s2.0-84880183607 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article MAGNETIC NANOPARTICLES OXIDATIVE STRESS DRUG DISCOVERY DIAGNOSTIC APPLICATIONS STROKE CANCER THERAPY DELIVERY GENE MICE Stroke Reactive oxygen species Ischemia-reperfusion Live imaging Nanoparticles