Singh, Ajay Lim, Chang-Keun Lee, Yong-Deok Maeng, Joon-ho Lee, Sangyoup Koh, Joonseok Kim, Sehoon 2024-01-20T11:32:28Z 2024-01-20T11:32:28Z 2022-01-10 2013-09-25 1944-8244 https://pubs.kist.re.kr/handle/201004/127655 Dyes showing solid-state fluorescence (SSF) are intriguing molecules that can emit bright fluorescence in the condensed phase. Because they do not suffer from self-quenching. of fluorescence, nanoscopic dense integration of those molecules produces particulate nanoprobes whose emission intensity can be boosted by raising the intraparticle dye density. In spite of the potential promise for imaging applications demanding intense emission signals, their excitation and emission spectra are generally limited to the visible region where biological tissues have less transparency. Therefore, the SSF-based nanoprobes have rarely been applied to noninvasive in vivo imaging. Here we report a combinatorial chemistry approach to attain a high level of tissue transparency of SSF by tuning its excitation and emission wavelengths to the truly near-infrared (NIR) region. We built a dipolar arylvinyl (ArV) scaffold-based chemical library where the optical bandgap could be narrowed to the NIR above 700 nm by combinatorial modulation of the g-electron push-pull strengths. The ArV-aggregated nanoparticles (FArV NPs) with a colloidal size less than 20 nm were formulated using a polymeric surfactant (Pluronic F-127) and applied to bioimaging in cells and in vivo. We demonstrate that some of FArV NPs have truly NIR excitation and emission of SSF, capable of noninvasive in vivo imaging (efficient lymph node mapping and early diagnosis of tumor) in mouse models by virtue of bright solid-state NW fluorescence and high signal-to-background contrast (S/B approximate to 8) as well as facile circulation in the living body. English American Chemical Society AGGREGATION-INDUCED EMISSION ORGANIC NANOPARTICLES ENHANCED FLUORESCENCE IN-VIVO DERIVATIVES LIGHT FLUOROPHORES BIOPROBES SYSTEMS PROBES Tuning Solid-State Fluorescence to the Near-Infrared: A Combinatorial Approach to Discovering Molecular Nanoprobes for Biomedical Imaging Article 10.1021/am4012066 1 ACS Applied Materials & Interfaces, v.5, no.18, pp.8881 - 8888 ACS Applied Materials & Interfaces 5 18 8881 8888 scie scopus 000330016500013 2-s2.0-84885036144 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Science & Technology - Other Topics Materials Science Article AGGREGATION-INDUCED EMISSION ORGANIC NANOPARTICLES ENHANCED FLUORESCENCE IN-VIVO DERIVATIVES LIGHT FLUOROPHORES BIOPROBES SYSTEMS PROBES aggregation-enhanced fluorescence biomedical in vivo imaging combinatorial chemistry dye-concentrated nanoparticles solid-state NIR fluorescence