Sharma, Shaili Lee, Aeju Choi, Kuiwon Kim, Kwangmeyung Youn, Inchan Trippel, Stephen B. Panitch, Alyssa 2024-01-20T11:33:34Z 2024-01-20T11:33:34Z 2021-09-05 2013-09 1616-5187 https://pubs.kist.re.kr/handle/201004/127711 Aggrecan, a major macromolecule in cartilage, protects the extracellular matrix (ECM) from degradation during the progression of osteoarthritis (OA). However, aggrecan itself is also susceptible to proteolytic cleavage. Here, the use of a biomimetic proteoglycan (mAGC) is presented, which functionally mimics aggrecan but lacks the known cleavage sites, protecting the molecule from proteolytic degradation. The objective of this study is to test the efficacy of this molecule in ex vivo (human OA synovial fluid) and in vivo (Sprague-Dawley rats) osteoarthritic models. These results indicate that mAGC's may protect articular cartilage against the loss of key ECM components, and lower catabolic protein and gene expression in both models. This suppression of matrix degradation has the potential to provide a healthy environment for tissue repair. English WILEY-V C H VERLAG GMBH HUMAN SYNOVIAL-FLUID INTERGLOBULAR DOMAIN OSTEOARTHRITIS DEVELOPMENT KNEE OSTEOARTHRITIS ARTICULAR-CARTILAGE HYALURONAN COLLAGEN PROTEOGLYCANS CHONDROCYTES EXPRESSION Biomimetic Aggrecan Reduces Cartilage Extracellular Matrix From Degradation and Lowers Catabolic Activity in Ex Vivo and In Vivo Models Article 10.1002/mabi.201300112 1 MACROMOLECULAR BIOSCIENCE, v.13, no.9, pp.1228 - 1237 MACROMOLECULAR BIOSCIENCE 13 9 1228 1237 scie scopus 000327882700011 2-s2.0-84884819295 Biochemistry & Molecular Biology Materials Science, Biomaterials Polymer Science Biochemistry & Molecular Biology Materials Science Polymer Science Article HUMAN SYNOVIAL-FLUID INTERGLOBULAR DOMAIN OSTEOARTHRITIS DEVELOPMENT KNEE OSTEOARTHRITIS ARTICULAR-CARTILAGE HYALURONAN COLLAGEN PROTEOGLYCANS CHONDROCYTES EXPRESSION aggrecan biomimetics cartilage extracellular matrix proteolytic enzymes