Trong Nhat Phan Wong, Ee Lin Sun, Xiaoyan Kim, Geunwoong Jung, Seung Hee Yoon, Chang No Yang, Beom-Seok 2024-01-20T11:33:43Z 2024-01-20T11:33:43Z 2021-09-05 2013-09 0916-8451 https://pubs.kist.re.kr/handle/201004/127718 Cell-surface expression of the discoidin domain receptor (DDR) tyrosine kinase family in high molecular mass form was controlled sensitively by the glucose concentration through a post-translational N-glycosylation process. Cycloheximide time-course experiments revealed that the high-molecular-mass forms of DDR1 and DDR2 were significantly less stable than control receptor tyrosine kinases. Site-directed mutational analysis of the consensus N-glycosylation sites of the DDRs revealed that mutations of asparagine 213 of DDR2 and asparagine 211 of DDR1, a conserved N-glycosylation site among vertebrate DDRs, inhibited the generation of the high-molecular-mass isoform. Taken together, these results suggest a mechanism to control the activity of the DDR family by regulating its cell-surface expression. Due to low stability, the steady-state population of functional DDR proteins in the cell surface depends sensitively on its maturation process via post-translational N-glycosylation, which is controlled by the glucose supply and the presence of a conserved N-glycosylation site. English TAYLOR & FRANCIS LTD EXTRACELLULAR-MATRIX COLLAGEN-BINDING DDR1 ACTIVATION GLYCAN KINASE ATHEROSCLEROSIS IDENTIFICATION PROLIFERATION INFLAMMATION Low Stability and a Conserved N-Glycosylation Site Are Associated with Regulation of the Discoidin Domain Receptor Family by Glucose via Post-Translational N-Glycosylation Article 10.1271/bbb.130351 1 BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.77, no.9, pp.1907 - 1916 BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 77 9 1907 1916 scie scopus 000326411900019 2-s2.0-84884651919 Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Chemistry, Applied Food Science & Technology Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Chemistry Food Science & Technology Article EXTRACELLULAR-MATRIX COLLAGEN-BINDING DDR1 ACTIVATION GLYCAN KINASE ATHEROSCLEROSIS IDENTIFICATION PROLIFERATION INFLAMMATION discoidin domain receptor glucose protein stability N-glycosylation asparagine