Ryu, Ju Hee Shin, Jung-Youn Kim, Sun Ah Kang, Sun-Woong Kim, Hyunjoon Kang, Seokyung Choi, Kuiwon Kwon, Ick Chan Kim, Byung-Soo Kim, Kwangmeyung 2024-01-20T11:33:51Z 2024-01-20T11:33:51Z 2021-09-05 2013-09 0142-9612 https://pubs.kist.re.kr/handle/201004/127725 Matrix metalloproteinase (MMP)-2 and MMP-9 have been known to play the role of essential mediators in angiogenesis. Non-invasive in vivo imaging approach using imaging probes is a potential method of detecting MMP activity in living animals, wherein imaging probes must include the characteristics of non-toxicity, specific targetability, and reasonable signal intensity. Here, we developed MMP-specific and self-quenched human serum albumin (HSA)-based (MMP-HSA) nanoprobes for non-invasive optical imaging of MMP activity during angiogenesis in the mouse hindlimb ischemia model. MMP-specific fluorogenic peptide probes, which were self-quenched with a near-infrared fluorophore and a quencher, were covalently conjugated to HSA (MMP-HSA nanoprobes). MMP-HSA nanoprobes formed stable nanoparticle structures of approximately 36 nm in diameter. Strongly self-quenched MMP-HSA nanoprobes boosted intense fluorescence signals in the presence of MMP-2 and MMP-9. Furthermore, MMP-HSA nanoprobes showed no cytotoxicity in cell culture. Importantly, intravenous injection of MMP HSA nanoprobes provided longer blood half-life and successful non-invasive optical imaging of MMP activity during angiogenesis in the mouse hindlimb ischemia model. In addition, the MMP activity visualized by MMP-HSA nanoprobes was consistent with the results of zymography, Western blot, and immunohistochemistry. MMP-HSA nanoprobes may be useful for monitoring of the initial process of angiogenesis through non-invasive MMP imaging. (C) 2013 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD HUMAN SERUM-ALBUMIN MMP ACTIVITY NANOPARTICLES SURFACE CANCER PROBE MATRIX-METALLOPROTEINASE-9 EXPRESSION MEMBRANE INJURY Non-invasive optical imaging of matrix metalloproteinase activity with albumin-based fluorogenic nanoprobes during angiogenesis in a mouse hindlimb ischemia model Article 10.1016/j.biomaterials.2013.05.074 1 BIOMATERIALS, v.34, no.28, pp.6871 - 6881 BIOMATERIALS 34 28 6871 6881 scie scopus 000322049200032 2-s2.0-84879461320 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article HUMAN SERUM-ALBUMIN MMP ACTIVITY NANOPARTICLES SURFACE CANCER PROBE MATRIX-METALLOPROTEINASE-9 EXPRESSION MEMBRANE INJURY Matrix metalloproteinase Albumin Molecular imaging Angiogenesis Fluorescence