Koo, Dong Chil Baek, So Yoon Jung, Sang Hoon Shim, Sang Hee 2024-01-20T11:33:58Z 2024-01-20T11:33:58Z 2021-09-04 2013-09 1226-8372 https://pubs.kist.re.kr/handle/201004/127731 Amethanolic extract of Dipsacus asper, having anti-diabetic activity, was examined as a possible aldose reductase (ALR2) inhibitor, a key enzyme involved in diabetic complications. Bioactivity guided fractionation led to the isolation of ten compounds, ursolic acid (1), oleanolic acid-3-O-alpha-L-arabinopyranoside (2), daucosterol (3), hederagenin-3-O-alpha-L-arabinopyranoside (4), sweroside(5), caffeic acid (6), esculetin (7), protocatechualdehyde (8), loganin (9), and vanilic acid (10) from the ethyl acetate fraction of D. asper methanol extract. Among them, compounds 4, 6, 7, and 8 exhibited inhibitory effects on aldose reductase, with IC50 values of 23.70, 16.71, 34.36, and 21.81 mu M, respectively. This is the first report on the isolation of these compounds from D. asper, and the ALR2 inhibitory activity of hederagenin-3-O-alpha-L-arabinopyranoside. These results suggest the successful use of the extract of D. asper for ameliorating diabetic complications. English KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING STRUCTURAL REQUIREMENTS DIABETIC-RATS GLYCATION LENS FLAVONOIDS ACID Aldose reductase inhibitory compounds from extracts of Dipsacus asper Article 10.1007/s12257-013-0173-z 1 BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.18, no.5, pp.926 - 931 BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 18 5 926 931 scie scopus kci ART001817144 000326040600012 2-s2.0-84887029782 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article STRUCTURAL REQUIREMENTS DIABETIC-RATS GLYCATION LENS FLAVONOIDS ACID Dipsacus asper aldose reductase hederagenin-3-O-alpha-L-arabinopyranoside esculetin