Kim, Eun-A Choi, Jiyoung Han, A. Reum Choi, Soo Young Hahn, Hoh-Gyu Cho, Sung-Woo 2024-01-20T11:34:00Z 2024-01-20T11:34:00Z 2021-09-04 2013-09 0161-813X https://pubs.kist.re.kr/handle/201004/127733 In a previous in vitro study, we demonstrated the protective effects of a new drug, 2-cyclopropylimino-3-methyl-1,3-thiazoline hydrochloride (KHG26377), against glutamate-induced excitotoxicity in cultured glial cells. In this study, we explored the possible mechanisms underlying the neuroprotective and anti-inflammatory effects of this compound against glutamate-induced excitotoxicity in rat brain. Our results showed that pretreatment with KHG26377 significantly attenuated glutamate-induced elevation of lipid peroxidation, TNF-alpha, IFN-gamma, nitric oxide, reactive oxygen species, NADPH oxidase, and Ca2+ levels, as well as the expression of caspase-3, neuronal nitric oxide synthase, and pERK. Furthermore, KHG26377 pretreatment attenuated key antioxidant parameters such as levels of superoxide dismutase, catalase, glutathione, glutathione peroxidase, and glutathione reductase, and also mitigated suppression of mitochondrial transmembrane potential by glutamate toxicity. Thus, through its antioxidant and anti-inflammatory activities in rat brain, KHG26377 may help protect against glutamate-induced neuronal damage. (C) 2013 Elsevier Inc. All rights reserved. English ELSEVIER ACTIVATED PROTEIN-KINASES SIGNAL-REGULATED KINASE NEURONAL CELL-LINE OXIDATIVE STRESS MITOCHONDRIAL DYSFUNCTION CEREBRAL-ISCHEMIA NADPH OXIDASE NITRIC-OXIDE PROVIDES NEUROPROTECTION MEDIATED NEUROTOXICITY Anti-oxidative and anti-inflammatory effects of 2-cyclopropylimino-3-methyl-1,3-thiazoline hydrochloride on glutamate-induced neurotoxicity in rat brain Article 10.1016/j.neuro.2013.07.001 1 NEUROTOXICOLOGY, v.38, pp.106 - 114 NEUROTOXICOLOGY 38 106 114 scie scopus 000325447800015 2-s2.0-84881303781 Neurosciences Pharmacology & Pharmacy Toxicology Neurosciences & Neurology Pharmacology & Pharmacy Toxicology Article ACTIVATED PROTEIN-KINASES SIGNAL-REGULATED KINASE NEURONAL CELL-LINE OXIDATIVE STRESS MITOCHONDRIAL DYSFUNCTION CEREBRAL-ISCHEMIA NADPH OXIDASE NITRIC-OXIDE PROVIDES NEUROPROTECTION MEDIATED NEUROTOXICITY Glutamate 2-Cyclopropylimino-3-methyl-1,3-thiazoline hydrochloride Reactive oxygen species Cytokines Calcium