Kim, S.-H. Yoo, H.H. Cha, E.-J. Jeong, E.S. Kim, H.J. Kim, D.H. Lee, J. 2024-01-20T11:34:11Z 2024-01-20T11:34:11Z 2021-09-02 2013-09 2233-4203 https://pubs.kist.re.kr/handle/201004/127743 An ultra-fast generic LC-MS/MS method was developed for high-throughput quantification of discovery pharmacokinetic (PK) samples and its reliability was verified. The method involves a simple protein precipitation for sample preparation and the analysis by ultra-fast generic LC-MS/MS with the ballistic gradient program and selected reaction monitoring (SRM) mode. Approximately 290 new chemical entities (NCEs) (over 10,000 samples) from 5 therapeutic programs were analyzed. The calibration curves showed good linearity in the concentration range of 1, 2 or 5 to 2000 ng/mL. No significant ion suppression was observed in the elution region of all the NCEs. When approximately 300 plasma samples were continuously analyzed, the peak area of internal standard was constant and reproducible. In the repeated analysis of samples, the plasma concentrations and the area under the curve (AUC) were consistent with the results from the first analysis. These results showed that the present ultra-fast generic LC-MS/MS method is reliable in terms of selectivity, sensitivity, and reproducibility and could be useful for high-throughput quantification and other bioanalysis in drug discovery. English Ultra-fast generic LC-MS/MS method for high-throughput quantification in drug discovery Article 10.5478/MSL.2013.4.3.47 1 Mass Spectrometry Letters, v.4, no.3, pp.47 - 50 Mass Spectrometry Letters 4 3 47 50 scopus ART001912271 2-s2.0-84884734853 Article Ballistic gradient system Drug discovery High-throughput quantification LC-MS/MS