Gary, Dana J. Min, Jungbin Kim, Youngwook Park, Keunchil Won, You-Yeon 2024-01-20T12:00:58Z 2024-01-20T12:00:58Z 2021-09-05 2013-08 1616-5187 https://pubs.kist.re.kr/handle/201004/127823 PEG-PnBA-PDMAEMA triblock and PEG-PDMAEMA diblock copolymers are used as model systems for studying the role of N/P ratio on the in vivo behavior of PEGylated siRNA carriers in mice. The presence of a free/uncomplexed polymer population coexisting with siRNA complexes is established. A change in the N/P ratio exerts no significant influence on the in vivo biodistribution and ex vivo blood chemistry properties of the respective systems. Histological analysis of major organs indicates that the presence of uncomplexed polymer elicits toxicity to the organ that is associated with the clearance of the siRNA complexes from the circulation system. This effect can be eliminated by working at N/P ratios near the charge-neutralization point of the complexes. English WILEY-V C H VERLAG GMBH GEL ELECTROPHORESIS POLYAMIDOAMINE DENDRIMERS POLYMERIC MICELLES DIBLOCK COPOLYMER GENE TRANSFECTION MOLECULAR-WEIGHT FREE POLYCATIONS DELIVERY BIODISTRIBUTION POLYPLEXES The Effect of N/P Ratio on the In Vitro and In Vivo Interaction Properties of PEGylated Poly[2-(dimethylamino)ethyl methacrylate]-Based siRNA Complexes Article 10.1002/mabi.201300046 1 MACROMOLECULAR BIOSCIENCE, v.13, no.8, pp.1059 - 1071 MACROMOLECULAR BIOSCIENCE 13 8 1059 1071 scie scopus 000327738700006 2-s2.0-84882691457 Biochemistry & Molecular Biology Materials Science, Biomaterials Polymer Science Biochemistry & Molecular Biology Materials Science Polymer Science Article GEL ELECTROPHORESIS POLYAMIDOAMINE DENDRIMERS POLYMERIC MICELLES DIBLOCK COPOLYMER GENE TRANSFECTION MOLECULAR-WEIGHT FREE POLYCATIONS DELIVERY BIODISTRIBUTION POLYPLEXES biodistribution blood toxicity N P ratio polyplexes siRNA