Gullotti, Emily Park, Joonyoung Yeo, Yoon 2024-01-20T12:01:08Z 2024-01-20T12:01:08Z 2021-09-05 2013-08 0724-8741 https://pubs.kist.re.kr/handle/201004/127831 To create poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs), where a drug-encapsulating NP core is covered with polyethylene glycol (PEG) in a normal condition but exposes a cell-interactive TAT-modified surface in an environment rich in matrix metalloproteinases (MMPs). PLGA NPs were modified with TAT peptide (PLGA-pDA-TAT NPs) or dual-modified with TAT peptide and a conjugate of PEG and MMP-substrate peptide (peritumorally activatable NPs, PANPs) via dopamine polymerization. Cellular uptake of fluorescently labeled NPs was observed with or without a pre-treatment of MMP-2 by confocal microscopy and flow cytometry. NPs loaded with paclitaxel (PTX) were tested against SKOV-3 ovarian cancer cells to evaluate the contribution of surface modification to cellular delivery of PTX. While the size and morphology did not significantly change due to the modification, NPs modified with dopamine polymerization were recognized by their dark color. TAT-containing NPs (PLGA-pDA-TAT NPs and PANPs) showed changes in surface charge, indicative of effective conjugation of TAT peptide on the surface. PLGA-pDA-TAT NPs and MMP-2-pre-treated PANPs showed relatively good cellular uptake compared to PLGA NPs, MMP-2-non-treated PANPs, and NPs with non-cleavable PEG. After 3 h treatment with cells, PTX loaded in cell-interactive NPs showed greater toxicity than non-interactive ones as the former could enter cells during the incubation period. However, due to the initial burst drug release, the difference was not as clear as microscopic observation. PEGylated polymeric NPs that could expose cell-interactive surface in response to MMP-2 were successfully created by dual modification of PLGA NPs using dopamine polymerization. English SPRINGER/PLENUM PUBLISHERS INTRACELLULAR DRUG-DELIVERY CELLULAR UPTAKE MATRIX METALLOPROTEINASES MAGNETIC NANOPARTICLES CANCER-THERAPY QUANTUM DOTS TAT PEPTIDE NANOCARRIERS PROTEIN FORMULATION Polydopamine-Based Surface Modification for the Development of Peritumorally Activatable Nanoparticles Article 10.1007/s11095-013-1039-y 1 PHARMACEUTICAL RESEARCH, v.30, no.8, pp.1956 - 1967 PHARMACEUTICAL RESEARCH 30 8 1956 1967 scie scopus 000321133100003 2-s2.0-84879795014 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article INTRACELLULAR DRUG-DELIVERY CELLULAR UPTAKE MATRIX METALLOPROTEINASES MAGNETIC NANOPARTICLES CANCER-THERAPY QUANTUM DOTS TAT PEPTIDE NANOCARRIERS PROTEIN FORMULATION dopamine polymerization PEG cleavage polymeric nanoparticles surface modification TAT peptide