Yun, Ji Ho Lee, Saet Byoul Kang, Kyungsu Lee, Eun Ha Lee, Hee Ju Jung, Sang Hoon Nho, Chu Won 2024-01-20T12:01:22Z 2024-01-20T12:01:22Z 2021-09-04 2013-08 1096-620X https://pubs.kist.re.kr/handle/201004/127842 Phase II detoxification enzymes are known to play essential roles in the detoxification and elimination of activated carcinogens during tumor initiation, while apoptosis is one of the most important chemopreventive targets for inhibiting tumor promotion in cancer. In this study, we investigated the cancer chemopreventive activity of two plant extracts, the ethanolic extract of Adenocaulon himalaicum (AHE) and the butanolic fraction of AHE (AHB). Both, the AHE and AHB induced NQO1 activity and had relatively high chemoprevention indices (CI = 12.4). The AHE and AHB were associated with increased NQO1 and HO-1 mRNA levels via Nrf2-ARE pathway activation. In addition, the AHB increased CYP1A1 activity through AhR-XRE pathway activation. We also found that the AHE and AHB induced apoptosis, as evidenced by phosphatidylserine externalization, an increase in the sub-G0/G1 content, chromatin condensation, poly(ADP-ribose) polymerase cleavage, and p53 induction. These data suggest that AHE and AHB act as bifunctional inducers and that their chemopreventive effects result from the biphasic induction of phase II detoxification enzymes and apoptosis. Therefore, these results suggest that A. himalaicum plant extracts have potential for use as chemopreventive agents for the prevention and/or treatment of human cancers. English MARY ANN LIEBERT, INC URINARY-BLADDER CARCINOGENESIS TRANSCRIPTIONAL REGULATION CANCER PREVENTION DIETARY PHYTOCHEMICALS PHENOLIC ANTIOXIDANTS INDUCIBLE EXPRESSION SIGNALING PATHWAY RESPONSE ELEMENT AH RECEPTOR CELLS Bifunctional Chemopreventive Effects of Adenocaulon himalaicum Through Induction of Detoxification Enzymes and Apoptosis Article 10.1089/jmf.2012.2625 1 JOURNAL OF MEDICINAL FOOD, v.16, no.8, pp.701 - 710 JOURNAL OF MEDICINAL FOOD 16 8 701 710 scie scopus kci ART001798227 000323430200004 2-s2.0-84883009685 Chemistry, Medicinal Food Science & Technology Nutrition & Dietetics Pharmacology & Pharmacy Food Science & Technology Nutrition & Dietetics Article URINARY-BLADDER CARCINOGENESIS TRANSCRIPTIONAL REGULATION CANCER PREVENTION DIETARY PHYTOCHEMICALS PHENOLIC ANTIOXIDANTS INDUCIBLE EXPRESSION SIGNALING PATHWAY RESPONSE ELEMENT AH RECEPTOR CELLS Adenocaulon himalaicum apoptosis colon cancer heme oxygenase Nrf2 quinone reductase