Bae, Woori Choi, Mal-Gi Hyeon, Changbong Shin, Yeon-Kyun Yoon, Tae-Young 2024-01-20T12:01:51Z 2024-01-20T12:01:51Z 2021-09-05 2013-07-17 0002-7863 https://pubs.kist.re.kr/handle/201004/127865 Current single-molecule techniques do not permit the real-time observation of multiple proteins interacting closely with each other. We here report an approach enabling us to determine the single-molecule fluorescence resonance energy transfer (FRET) kinetics of multiple protein-protein interactions occurring far below the diffraction limit. We observe a strongly cooperative formation of multimeric soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes, which suggests that formation of the first SNARE complex triggers a cascade of SNARE complex formation. English AMER CHEMICAL SOC MEMBRANE-FUSION DYNAMICS RESOLUTION MECHANISM ANATOMY SNARES Real-Time Observation of Multiple-Protein Complex Formation with Single-Molecule FRET Article 10.1021/ja404276g 1 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.135, no.28, pp.10254 - 10257 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135 28 10254 10257 scie scopus 000322103000019 2-s2.0-84880360847 Chemistry, Multidisciplinary Chemistry Article MEMBRANE-FUSION DYNAMICS RESOLUTION MECHANISM ANATOMY SNARES